Use of a white light supercontinuum laser for confocal interference-reflection microscopy.

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  • Author(s): CHIU, L.-D.; SU, L.; REICHELT, S.; AMOS, W.B.
  • Source:
    Journal of Microscopy. May2012, Vol. 246 Issue 2, p153-159. 7p. 1 Color Photograph, 2 Black and White Photographs, 1 Diagram, 2 Graphs.
  • Additional Information
    • Subject Terms:
      INTERFERENCE microscopy; CONFOCAL microscopy; LASERS; FLUORESCENCE microscopy; OPTICAL reflection; INTERFEROMETERS; LIGHT sources
    • Abstract:
      Shortly after its development, the white light supercontinuum laser was applied to confocal scanning microscopy as a more versatile substitute for the multiple monochromatic lasers normally used for the excitation of fluorescence. This light source is now available coupled to commercial confocal fluorescence microscopes. We have evaluated a supercontinuum laser as a source for a different purpose: confocal interferometric imaging of living cells and artificial models by interference reflection. We used light in the range 460-700 nm where this source provides a reasonably flat spectrum, and obtained images free from fringe artefacts caused by the longer coherence length of conventional lasers. We have also obtained images of cytoskeletal detail that is difficult to see with a monochromatic laser. [ABSTRACT FROM AUTHOR]