[Role of lysines in the stability of a snake neurotoxin].

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  • Author(s): Ménez A; Tamiya N
  • Source:
    Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie [C R Seances Acad Sci III] 1982 May 24; Vol. 294 (19), pp. 957-62.
  • Publication Type:
    English Abstract; Journal Article
  • Language:
    French
  • Additional Information
    • Transliterated Title:
      Rôle des lysines dans la stabilité d'une neurotoxine de serpent.
    • Source:
      Publisher: Gauthier Villars Country of Publication: France NLM ID: 8108553 Publication Model: Print Cited Medium: Print ISSN: 0249-6313 (Print) Linking ISSN: 02496313 NLM ISO Abbreviation: C R Seances Acad Sci III Subsets: MEDLINE
    • Publication Information:
      Publication: Montreuil : Gauthier Villars
      Original Publication: Paris : Gauthier-Villars, c1981-[1983]
    • Subject Terms:
      Elapid Venoms* ; Erabutoxins* ; Lysine*; Acylation ; Animals ; Circular Dichroism ; Drug Stability ; Guanidine ; Guanidines ; Protein Conformation ; Snakes ; Thermodynamics
    • Abstract:
      The effects of charge suppression at a single lysine residue on the energetics of thermal unfolding or unfolding with guanidinium chloride of erabutoxin b are studied by circular dichroism. It is shown that acylation of lysine 15, 47 or 51 has virtually no effect on the toxin stability. In contrast, abolition of the positive charge of lys-27, a residue involved in the "toxic" site of the molecule, substantially increases the toxin stability. This phenomenon is attributed to suppression of repulsive interactions occurring within the native toxin molecule between lys-27 and other positively charged groups.
    • Accession Number:
      0 (Elapid Venoms)
      0 (Guanidines)
      59536-69-5 (Erabutoxins)
      9083-23-2 (erabutoxin b)
      JU58VJ6Y3B (Guanidine)
      K3Z4F929H6 (Lysine)
    • Publication Date:
      Date Created: 19820524 Date Completed: 19830119 Latest Revision: 20131121
    • Publication Date:
      20240829
    • Accession Number:
      6814715