Increased satellite association induced by 5' bromodeoxyuridine treatment of phytohemaglutinin-stimulated blood lymphocytes.

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  • Additional Information
    • Source:
      Publisher: Springer Verlag Country of Publication: Germany NLM ID: 7613873 Publication Model: Print Cited Medium: Print ISSN: 0340-6717 (Print) Linking ISSN: 03406717 NLM ISO Abbreviation: Hum Genet Subsets: MEDLINE
    • Publication Information:
      Publication: Berlin : Springer Verlag
      Original Publication: Berlin, New York, Springer-Verlag.
    • Subject Terms:
      Bromodeoxyuridine/*pharmacology ; Chromosomes/*ultrastructure ; DNA, Satellite/*analysis ; Lymphocytes/*ultrastructure ; Nucleolus Organizer Region/*ultrastructure; Adolescent ; Adult ; Centromere ; Humans ; Lymphocytes/drug effects ; Metaphase ; Phytohemagglutinins/pharmacology
    • Abstract:
      5'-Bromodeoxyuridine (BrdU) present in the course of late S and G2 phases of the cell cycle in PHA-stimulated human lymphocyte cultures causes the despiralization and elongation of some chromosome regions, including short arms of acrocentric chromosomes. BrdU present at a concentration of 250 microM during the last 10 h in lymphocyte cultures from 19 healthy subjects did not affect the number of silver-stained NORs, but raised significantly the number of satellite associations of acrocentric chromosomes. The mere substitution of thymine by BrdU in DNA strands as a reason for increased number of satellite associations seems a less plausible explanation than the modification of DNA-protein complexes of NOR regions, which could alter the degree of their spiralization and cause the increased tendency of acrocentric chromosomes to associate in the subsequent metaphase.
    • References:
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    • Accession Number:
      0 (DNA, Satellite)
      0 (Phytohemagglutinins)
      G34N38R2N1 (Bromodeoxyuridine)
    • Publication Date:
      Date Created: 19830101 Date Completed: 19840215 Latest Revision: 20190722
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/BF00286640
    • Accession Number:
      6654340