Efecto de glucosa en la expresión de lipasa endotelial en células endoteliales humanas y en sujetos con diabetes mellitus tipo 2.

GLUCOSE EFFECT IN THE EXPRESSION OF ENDOTHELIAL LIPASE IN HUMAN ENDOTHELIAL CELLS AND IN PATIENTS WITH DIABETES MELLITUS TYPE 2.

LIPASES; HIGH density lipoproteins; INFLAMMATION; CYTOKINES; GLUCOSE; DIABETES; METABOLIC regulation

Introducción: Lipasa endotelial (LE), enzima que modula el metabolismo de HDL, es sobreregulada por citoquinas-inflamatorias. Diabetes mellitus tipo 2 (DM2) se ha asocia a inflamación subclínica, por lo que se plantea que estos pacientes tendrían niveles elevados de LE. El objetivo del estudio es determinar el efecto de glucosa en expresión de LE en células de cultivo y evaluar la relación entre los niveles de LE y el control glicémico en sujetos con DM2. Método: Células endoteliales humanas (HUVEC) fueron estimuladas con distintas concentraciones de glucosa (5.5, 25 y 50 mmol/L) durante 24 h, se evaluó el efecto sobre la expresión de LE. En sujetos DM2 se midieron niveles de LE, glicemia y hemoglobina glicosilada fracción Ale (HbA1c). Se contó con un grupo control (8) para la determinación de los niveles de la enzima. LE se midió por inmunotransferencia, y los resultados fueron expresados como unidades arbitrarias(UA). Resultados: En células HUVEC la expresión de LE fue directamente proporcional a la concentración de glucosa extracelular (p < 0,05). Se evaluaron 24 sujetos diabéticos (15 mujeres y 9 hombres), edad promedio 60 ± 9,7años, que presentaron niveles de LE mayores que el grupo control (14911UA y 10250,18UA respectivamente, p < 0,05). No se encontró relación entre glicemia, HbA1c y LE. Conclusión: En células HUVEC existe relación directa entre glucosa extracelular y LE. Los sujetos diabéticos tuvieron niveles mayores de LE que el grupo control, pero esto no se relacionó con control glicémico, lo que apunta a la existencia de otros factores que participen en el aumento de la expresión de LE. [ABSTRACT FROM AUTHOR]

Introduction: Endothelial Lipase (EL), enzyme that modulates HDL metabolism, is overregulated by inflammatory-cytokines. Type 2 Diabetes (DM2) has been associated with a subclinical inflammation, so it has been ruled that these patients could have high levels of EL. The objectives of the research are to determine the effect of glucose in the expression of EL in culturing cells and evaluate the relation between the levels of EL and the metabolic control in patients with DM2. Method: During 24 hours, human endothelial cells (HUVEC) were stimulated with different concentrations of glucose (5.5, 25 and 50 mmol/L), the effect was evaluated over the expression of EL. In DM2 patients levels of EL, glucose and HbA1c were measured. We had a control group (8) to determine the levels of enzyme. EL was measured by immune transference, and the results were expressed by arbitrary units(AU). Results: In HUVEC cells, the expression of EL was directly proportional extracellular glucose (p < 0.05). 24 diabetic patients were evaluated (15 females and 9 males) average age from 60 ± 9,7 years old. The studied group showed levels of EL bigger than the control group (14911 AU and 10250,18AU) respectively (p < 0.05). We found no relation between glucose, HbA1c and EL. Conclusion: In HUVEC cells there is a direct relation between extracell glucose and EL. The diabetic patients had higher levels of EL than the control group, but these was not related with glucose or HbA1c, these shows the existence of other factors that participate in the increasement of EL. [ABSTRACT FROM AUTHOR]

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