Detection of K+ and Cl-channels from calf cardiac sarcolemma in planar lipid bilayer membranes.

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  • Author(s): Coronado R; Latorre R
  • Source:
    Nature [Nature] 1982 Aug 26; Vol. 298 (5877), pp. 849-52.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print Cited Medium: Print ISSN: 0028-0836 (Print) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
    • Publication Information:
      Publication: Basingstoke : Nature Publishing Group
      Original Publication: London, Macmillan Journals ltd.
    • Subject Terms:
      Chlorides/*metabolism ; Ion Channels/*metabolism ; Myocardium/*metabolism ; Potassium/*metabolism ; Sarcolemma/*metabolism; Action Potentials ; Animals ; Calcium/metabolism ; Cattle ; Lipid Bilayers/metabolism ; Purkinje Fibers/metabolism ; Sodium/metabolism
    • Abstract:
      The ionic currents underlying the cardiac action potential are believed to be much more complex than those in nerve. During the cardiac action potential, various membrane channels control the flow of K+, Na+, Ca2+ and Cl- across the sarcolemma of cardiac muscle cells. Thus, it has become increasingly clear that a detailed knowledge of the mechanisms that activate (or inactivate) heart channels is required to understand cardiac excitability. We report here the use of planar lipid bilayer techniques to detect and characterize K+ and Cl- channels in purified heart sarcolemma membrane vesicles. We have identified four different types of channel on the basis of their selectivity, conductance and gating kinetics. We present in some detail the properties of a K+ channel and a Cl- channel. We have tentatively identified the K+ channel with the ix type of current found in Purkinje, myocardial ventricular and atrial fibres. The chloride channel might be related to the transient chloride current found in Purkinje fibres.
    • Grant Information:
      GM-25277 United States GM NIGMS NIH HHS; GM-28992 United States GM NIGMS NIH HHS
    • Accession Number:
      0 (Chlorides)
      0 (Ion Channels)
      0 (Lipid Bilayers)
      9NEZ333N27 (Sodium)
      RWP5GA015D (Potassium)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 19820826 Date Completed: 19821012 Latest Revision: 20190617
    • Publication Date:
      20221213
    • Accession Number:
      10.1038/298849a0
    • Accession Number:
      6287275