Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning.

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    • Subject Terms:
      GALACTOSYLTRANSFERASES; TRANSPLANTATION of cell nuclei; CLONING
    • Abstract:
      The presence of galactose α-l,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes. [ABSTRACT FROM AUTHOR]
    • Abstract:
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