Radiation damage to bull sperm motility. II. Proton irradiation and respiration measurements.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Rikmenspoel R; van Herpen G
  • Source:
    Biophysical journal [Biophys J] 1969 Jun; Vol. 9 (6), pp. 833-44.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print Cited Medium: Print ISSN: 0006-3495 (Print) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE
    • Publication Information:
      Publication: Cambridge, MA : Cell Press
      Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society.
    • Subject Terms:
      Cell Biology* ; Protons* ; Radiation Effects*; Oxygen Consumption/*radiation effects ; Spermatozoa/*radiation effects; Adenosine Triphosphate/metabolism ; Animals ; Cattle ; Cell Movement ; Flagella/radiation effects ; Male ; Oxidative Phosphorylation/radiation effects
    • Abstract:
      Diluted bull semen samples were bombarded with a 24 Mev proton beam. Dose response curves for the fraction of cells which survived the bombardment and for the average velocity of the surviving cells were measured. Target theory indicated a cross section of the sensitive volume of 2.1 x 10(-10) cm(2). Respiration measurements showed that the oxidative phosphorylation in the sperm remained coupled after the bombardments. The efficiency with which free energy from ATP hydrolysis was converted into mechanical work by the sperm was found to decrease after proton bombardment. The half-value dose for this effect was two and a half times higher than the half-value dose for motility damage. These respiration measurements indicate that the damage due to the bombardment is not to the metabolic system or to the contractile system in the sperm flagellum, but to a control system for the motility. The results of the target theory shows that this control system is localized in a small element of approximately 1600 A diameter. The centriole is tentatively proposed as being this control element.
    • References:
      Exp Cell Res. 1965 Feb;37:312-26. (PMID: 14298944)
      Arch Biochem Biophys. 1958 Dec;78(2):587-97. (PMID: 13618041)
      Am J Anat. 1964 Jul;115:163-83. (PMID: 14199781)
      Biophys J. 1965 Jul;5(4):365-92. (PMID: 5893155)
      Exp Cell Res. 1965 May;38:217-46. (PMID: 14284505)
      J Gen Physiol. 1968 Aug;52(2):283-99. (PMID: 4234210)
      Phys Med Biol. 1960 Oct;5:167-81. (PMID: 13741673)
    • Accession Number:
      0 (Protons)
      8L70Q75FXE (Adenosine Triphosphate)
    • Publication Date:
      Date Created: 19690601 Date Completed: 19690830 Latest Revision: 20181113
    • Publication Date:
      20240829
    • Accession Number:
      PMC1367479
    • Accession Number:
      10.1016/S0006-3495(69)86421-0
    • Accession Number:
      5794110