Precipitation of proteins by vinblastine and calcium ions.

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  • Author(s): Wilson L; Bryan J; Ruby A; Mazia D
  • Source:
    Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1970 Jul; Vol. 66 (3), pp. 807-14.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : National Academy of Sciences
    • Subject Terms:
      Brain Chemistry* ; Calcium* ; Chemical Precipitation* ; Vinblastine*; Nerve Tissue Proteins/*isolation & purification ; Organoids/*analysis; Animals ; Blood Proteins ; Calcium Isotopes ; Chick Embryo ; Chickens ; Colchicine ; Erythrocytes ; Indicators and Reagents ; Lactoglobulins/isolation & purification ; Microtubules/analysis ; Muramidase/isolation & purification ; Muscle Proteins/isolation & purification ; Protein Binding ; Serum Albumin, Bovine/isolation & purification ; Tritium
    • Abstract:
      We have found that vinblastine sulfate can precipitate a number of proteins in addition to microtubule protein. Those proteins which precipitate with vinblastine sulfate, all of which were derived from structure, also precipitate with Ca(2+) ions. Our results suggest that vinblastine sulfate, presumably acting as a cation, precipitates proteins by combining with sites which can also combine with Ca(2+) ions.
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    • Accession Number:
      0 (Blood Proteins)
      0 (Calcium Isotopes)
      0 (Indicators and Reagents)
      0 (Lactoglobulins)
      0 (Muscle Proteins)
      0 (Nerve Tissue Proteins)
      10028-17-8 (Tritium)
      27432CM55Q (Serum Albumin, Bovine)
      5V9KLZ54CY (Vinblastine)
      EC 3.2.1.17 (Muramidase)
      SML2Y3J35T (Colchicine)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 19700701 Date Completed: 19700821 Latest Revision: 20190501
    • Publication Date:
      20221213
    • Accession Number:
      PMC283122
    • Accession Number:
      10.1073/pnas.66.3.807
    • Accession Number:
      5269244