Hypertonic cryohemolysis of human red blood cells.

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  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: United States NLM ID: 0211301 Publication Model: Print Cited Medium: Print ISSN: 0022-2631 (Print) Linking ISSN: 00222631 NLM ISO Abbreviation: J Membr Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, Springer.
    • Subject Terms:
      Cold Temperature* ; Erythrocytes*/drug effects ; Erythrocytes*/metabolism ; Hemolysis*/drug effects; Adenosine Triphosphate/blood ; Anesthetics/pharmacology ; Blood Proteins/metabolism ; Humans ; In Vitro Techniques ; Membrane Proteins/metabolism ; Saline Solution, Hypertonic ; Spectrin/metabolism
    • Abstract:
      Hypertonic cryohemolysis of human erythrocytes is caused by incubation of the cells in hypertonic medium at a temperature of 20--50 degrees C (stage 1), with subsequent cooling to 0 degrees C (stage 2). In 0.86 M sucrose hemolysis increases, with increasing stage 1 temperature, whereas in 1 M NaCl cryohemolysis has a temperature optimum at a stage 1 temperature of about 30 degrees C. Cryohmeolysis is inhibited by preceding ATP depletion of the cells and by preincubation of the cells in hypertonic medium at 0 degrees C. In general, anesthetics inhibit cryohemolysis strongly. Only in 1 M NaCl at stage 1 temperatures in the range of 40--50 degrees C is cryohemolysis stimulated by these drugs, if present during the entire incubation period. This effect is abolished, however, when the anesthetic is added after prior incubation of the cells at 40--50 degrees C in 1 M NaCl. Ghost-bound ANS fluorescence indicates complicated conformation changes in the membrane structure during the various experimental stages leading to cryohemolysis. Some of the experimental results can be considered as examples of molecular hysteresis, thus indicating several different metastable structures of the membrane, under various experimental conditions. The described results support the working hypothesis of Green and Jung that the experimental procedure results in membrane protein damage, preventing normal adaptation of the membrane during cooling.
    • References:
      Biochemistry. 1971 Jun 22;10(13):2606-17. (PMID: 4326772)
      Cryobiology. 1977 Jun;14(3):287-302. (PMID: 891224)
      Biochemistry. 1973 Sep 11;12(19):3662-7. (PMID: 4274720)
      FEBS Lett. 1969 Apr;3(2):150-152. (PMID: 11946993)
      Annu Rev Biophys Bioeng. 1974;3(0):341-63. (PMID: 4609398)
      J Clin Invest. 1963 Apr;42:581-8. (PMID: 13999462)
      Biochim Biophys Acta. 1953 Mar;10(3):414-26. (PMID: 13058999)
      J Biol Chem. 1977 Apr 25;252(8):2753-63. (PMID: 15998)
      Biochemistry. 1974 Jan 1;13(1):171-8. (PMID: 4586935)
      Biochim Biophys Acta. 1970 Mar 17;203(1):67-76. (PMID: 5445681)
      J Membr Biol. 1971 Jun;5(2):154-68. (PMID: 24173098)
      Biochim Biophys Acta. 1975 Oct 17;406(3):415-23. (PMID: 241415)
      Life Sci. 1977 Aug 1;21(3):433-40. (PMID: 895375)
      Acta Physiol Scand. 1968 Jul;73(3):341-64. (PMID: 5709591)
      Biochem Pharmacol. 1968 Oct;17(10):2215-23. (PMID: 5696887)
      Biochemistry. 1969 Jul;8(7):2742-7. (PMID: 5808332)
      Proc R Soc Lond B Biol Sci. 1957 Dec 17;147(929):427-33. (PMID: 13494456)
      Cryobiology. 1975 Jun;12(3):192-201. (PMID: 1132235)
      Proc Natl Acad Sci U S A. 1976 Oct;73(10):3558-61. (PMID: 10571)
      J Membr Biol. 1978 Mar 10;39(2-3):273-84. (PMID: 25342)
      Biochem Biophys Res Commun. 1977 Aug 8;77(3):811-7. (PMID: 901514)
      J Membr Biol. 1977 May 12;33(3-4):249-62. (PMID: 864690)
      Biochem Pharmacol. 1979;28(2):177-82. (PMID: 426832)
      Biochem J. 1970 Feb;116(4):721-31. (PMID: 5435498)
      Biochem Biophys Res Commun. 1973 Dec 19;55(4):1240-5. (PMID: 4793159)
      Nature. 1954 Apr 10;173(4406):659-61. (PMID: 13165618)
      Z Naturforsch C. 1977 Jul-Aug;32(7-8):627-31. (PMID: 143824)
      Br J Haematol. 1955 Jan;1(1):117-29. (PMID: 13240001)
      Arch Biochem Biophys. 1973 Jun;156(2):604-12. (PMID: 4352419)
      Pharmacol Rev. 1972 Dec;24(4):583-655. (PMID: 4565956)
      J Cell Biol. 1974 Jul;62(1):1-19. (PMID: 4600883)
      Biochemistry. 1977 Jul 26;16(15):3450-4. (PMID: 889805)
      J Clin Invest. 1978 Mar;61(3):815-27. (PMID: 25286)
    • Accession Number:
      0 (Anesthetics)
      0 (Blood Proteins)
      0 (Membrane Proteins)
      0 (Saline Solution, Hypertonic)
      12634-43-4 (Spectrin)
      8L70Q75FXE (Adenosine Triphosphate)
    • Publication Date:
      Date Created: 19791130 Date Completed: 19800226 Latest Revision: 20190819
    • Publication Date:
      20221208
    • Accession Number:
      10.1007/BF01868890
    • Accession Number:
      513114