[Method of lysing animal cells grown on slides on top of an alkaline saccharose gradient].

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  • Author(s): Khokhlov AN; Vilenchik MM
  • Source:
    Tsitologiia [Tsitologiia] 1979 Aug; Vol. 21 (8), pp. 986-8.
  • Publication Type:
    English Abstract; Journal Article
  • Language:
    Russian
  • Additional Information
    • Transliterated Title:
      Metodika lizisa kletok zhivotnykh, vyrashchennykh na predmetnykh steklakh, na vershine gradienta shchelochnoĭ sakharozy.
    • Source:
      Publisher: Rossiiskaia Akademiia Nauk Country of Publication: Russia (Federation) NLM ID: 0417363 Publication Model: Print Cited Medium: Print ISSN: 0041-3771 (Print) Linking ISSN: 00413771 NLM ISO Abbreviation: Tsitologiia Subsets: MEDLINE
    • Publication Information:
      Publication: Moskva : Rossiiskaia Akademiia Nauk
      Original Publication: Moskva, Akademiia nauk SSSR.
    • Subject Terms:
      Cell Fractionation/*methods ; Centrifugation, Density Gradient/*methods; Animals ; Cell Fractionation/instrumentation ; Cells, Cultured ; Centrifugation, Density Gradient/instrumentation ; DNA Repair ; Diploidy ; Fibroblasts/cytology ; Humans
    • Abstract:
      A device is described providing for lysis of cells grown on glass slides, directly on the top of sucrose gradient. The device allows to avoid cell removing with trypsin, EDTA or due to scraping. The possibility to use this method in studying DNA damage and repair processes in cultured animal cells has been shown using diploid human fibroblasts.
    • Publication Date:
      Date Created: 19790801 Date Completed: 19791218 Latest Revision: 20061115
    • Publication Date:
      20240829
    • Accession Number:
      494396