Detekcija i tipizacija humanih papiloma virusa metodom polimorfizma restrikcijskih fragmenata u žena s razlićitim citološkim nalazom.

Detection and typing of human papillomaviruses by restriction fragment length polymorphism in women with different cytology.

Aim The aim of this study was to determine a percentage of high risk genotypes which are included in the current vaccines in women with different cytology and on the basis of obtained results to evaluate the protective activity of the current vaccines. Methods Endocervical swabs were taken from 70 women with different cervical cytology (25 women with CIN 1, 25 women with CIN 2/3 and 20 women with negative cytology). The samples were tested by PCR method using MY09/MY11 primers to determinate the HPV status, especially the percentage of high risk genotypes which are not included in the current vaccines. Genotyping was done by RFLP method. Results In as much as 3 8.8% of all samples high risk genotypes (hrHPV), not included in the current vaccine, were detected; in a group of women with normal cytology hrHPV genotypes were found in 29.9% samples. HrHPV were found in 47%, and 64% of samples taken from women with normal cytology and CIN 1, respectively, while in women with CIN 2/3 hrHPV were found in 94%. Conclusion These results indicate that in more than in one third of tested women the current available vaccines would be of minimal protective activity, but further studies which should include more women are needed. [ABSTRACT FROM AUTHOR]

Cilj ovoga rada bioje istražiti postotak genotipova visokog rizika, koji nisu uključeni u vakcinu trenutno doslupnu na tržištu, u žena s različitim citološkim nalazima, te na temelju učestalosti pojedinih genotipova, procijeniti zaštitnu ulogu dostupnih cjepiva. Metode: U istraživanjeje uključeno 70 žena s različitim citološkini nalazima (25 žena s CIN 1, 25 žena s CIN 2/3 i 20 žena s urednim citološkim nalazom) kojima je iz obrisaka vrata maternice PCR reakcijom, uz začetnice MY09/MY11, dokazana prisutnost DNA HPV-a. Genotipizacija HPV-a napravijenaje RFLP metodom. Rezultati: U čak 38,8% uzoraka svih skupina žena nađeni su genotipovi visokog rizika koji nisu uključeni u trenutno dostupne vakcine; u skupini žena s urednim citološkim nalazorn, ovi genotipovi nađeni su u čak 29,9% uzoraka. Dokazani su genotipovi visokog rizika u 47% uzoraka žena s normalnim citološkim nalazom, dok su u žena s CIN 1 nađeni u 64%, a u žena s CIN 2/3 u 94% uzoraka. Zaključak: Navedeni rezultati upućuju da bi kod više od trečine ispitanica postojeća cjepiva bila minirnalne djelotvornosti, no potrebna su daljnja istraživanja koja bi uključila veći broj ispitanica. [ABSTRACT FROM AUTHOR]

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