Metabolism of 25-hydroxyvitamin D3 in anephric rats: a new active metabolite.

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  • Author(s): Garabedian M; Pavlovitch H; Fellot C; Balsan S
  • Source:
    Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1974 Feb; Vol. 71 (2), pp. 554-7.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : National Academy of Sciences
    • Subject Terms:
    • Abstract:
      After the injection of radioactive 25-hydroxyvitamin D(3) into rats given a normal diet, two metabolites can be detected on Sephadex LH-20 chromatography modified to reveal polar metabolites. The first is designated peak Va, which by means of periodate cleavage has been identified as 24,25-dihydroxyvitamin D(3). The second more polar metabolite has been designated peak X(1) and is only partially sensitive to periodate. This metabolite appears in serum, bone, intestine, and liver and does not chromatograph on any system like any of the previously identified metabolites. Nephrectomy, especially in the animals given large doses of 25-hydroxyvitamin D(3), does not abolish production of either peak Va or X(1), revealing that both metabolites can be made in extra-renal tissue. Peak X(1) has marked ability to elevate serum calcium of rats on a vitamin D-deficient, low calcium diet, but requires the presence of kidneys to carry out this function.
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    • Accession Number:
      0 (Dihydroxycholecalciferols)
      0 (Hydroxycholecalciferols)
      10028-17-8 (Tritium)
    • Publication Date:
      Date Created: 19740201 Date Completed: 19740501 Latest Revision: 20190501
    • Publication Date:
      20231215
    • Accession Number:
      PMC388046
    • Accession Number:
      10.1073/pnas.71.2.554
    • Accession Number:
      4521821