Structural elucidation and evaluation of toxicity and antitumor activity of cardiac glycosides isolated from Leptadenia pyrotechnica.

GLYCOSIDES; NUCLEAR magnetic resonance; HIGH performance liquid chromatography; SPECTRUM analysis; FRESHWATER animals

Investigation of the chemical constituents of Leptadenia pyrotechnica (Forsk.) Decne (Asclepiadaceae) led to the isolation of three cardiac glycosides; 14,19-dihydroxycard-20 (22)-enolide-3- O-[ β-d-glucopyranosyl- β-d-digitoxoside] C-I, 14,19-dihydroxycard-20 (22)-enolide-3- O-[ β-d-glucopyranosyl- β-d-glucopyranoside] C-II and 14,19-dihydroxycard-20 (22)-enolide-3- O- β-d-digitoxoside C-III. The isolation and identification of these compounds were carried out using rotation locular counter current chromatography (RLCCC), high performance liquid chromatography (HPLC). The structures of the isolated compounds were established by fast-atom bombardment (FAB) and electrospray ionization (ESI) mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopic analysis. The acute toxicity studies of the cardiac glycosides and total alcohol extracts were examined by brine shrimp. Their lethal concentration 50 (LC50) were 18.84 and 11.89 ppm, respectively. The antitumor activity potato disk assays of the cardiac glycosides and total alcohol extracts had shown good activity − 30.8% and 49.3%, respectively. [ABSTRACT FROM AUTHOR]

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