Quantitative measurement of precipitating antibodies and standardization of Streptococcus group B antisera by the reverse radial immunodiffusion technique.

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  • Author(s): George JR; Harrell WK
  • Source:
    Bulletin of the World Health Organization [Bull World Health Organ] 1974; Vol. 51 (3), pp. 245-8.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: World Health Organization Country of Publication: Switzerland NLM ID: 7507052 Publication Model: Print Cited Medium: Print ISSN: 0042-9686 (Print) Linking ISSN: 00429686 NLM ISO Abbreviation: Bull World Health Organ Subsets: MEDLINE
    • Publication Information:
      Original Publication: Geneva : World Health Organization,
    • Subject Terms:
      Antibodies, Bacterial/*analysis ; Immune Sera/*analysis ; Immunodiffusion/*methods ; Precipitin Tests/*standards ; Streptococcus agalactiae/*immunology; Animals ; Carbohydrates ; Immunoelectrophoresis ; Precipitins/analysis ; Rabbits
    • Abstract:
      A comparative study was made of the reverse radial immunodiffusion (RRID) technique and the quantitative precipitin test for determining the amount of precipitable antibodies present in streptococcus group B antisera. The coefficient of correlation between the 2 tests was 0.963 when a purified carbohydrate antigen was used. The results of this study show that: (1) a group B antiserum should contain at least 3 mg of precipitable antibodies per ml to ensure that it will react with acid extracts of group B diagnostic cultures; and (2) the RRID technique is an accurate and simple procedure for determining the amount of precipitable antibody present in an antiserum, and it could be used for the standardization of these diagnostic reagents. A method for preparing the group B carbohydrate antigen used in the RRID and quantitative precipitin tests is described.
    • References:
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      J Exp Med. 1964 Oct 1;120:629-37. (PMID: 14212124)
      J Bacteriol. 1965 Feb;89:398-402. (PMID: 14255706)
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    • Accession Number:
      0 (Antibodies, Bacterial)
      0 (Carbohydrates)
      0 (Immune Sera)
      0 (Precipitins)
    • Publication Date:
      Date Created: 19740101 Date Completed: 19750913 Latest Revision: 20181113
    • Publication Date:
      20221208
    • Accession Number:
      PMC2366285
    • Accession Number:
      4218968