Some factors affecting the viability of dried bacteria during storage in vacuo.

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  • Author(s): Marshall BJ; Coote GG; Scott WJ
  • Source:
    Applied microbiology [Appl Microbiol] 1974 Apr; Vol. 27 (4), pp. 648-52.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 7605802 Publication Model: Print Cited Medium: Print ISSN: 0003-6919 (Print) Linking ISSN: 00036919 NLM ISO Abbreviation: Appl Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington DC : American Society For Microbiology
    • Subject Terms:
      Freeze Drying* ; Preservation, Biological*; Pseudomonas fluorescens/*metabolism ; Salmonella/*metabolism; Alanine/pharmacology ; Bacteriological Techniques ; Carbon Radioisotopes ; Cell Count ; Cell Survival/drug effects ; Factor Analysis, Statistical ; Glutamates/pharmacology ; Lysine/pharmacology ; Ribose/metabolism ; Ribose/pharmacology ; Semicarbazides/pharmacology ; Stereoisomerism ; Sucrose/pharmacology ; Temperature ; Time Factors ; Water
    • Abstract:
      The effects of various substances on the viability of freeze-dried cells of Pseudomonas fluorescens and Salmonella newport were studied during storage in vacuo for 5 years. Mixtures of two organisms were dried together and studied in two factorial experiments. The first was a complete factorial using six factors and two levels; the second was a fractional replicate with four factors at two levels and three others at four concentrations. A study of ribose binding by cells of S. newport was made by using [U-(14)C]ribose. Substantial improvements in viability were obtained by drying in the presence of sucrose, glutamate, and semicarbazide. Low concentrations of ribose increased the death rate during drying, but these adverse effects were prevented by equimolar amounts of semicarbazide. Ribose binding increased with storage time and, although its incorporation changed, most of the increase in total ribose occurred after the main decrease in viability. Storage temperature caused larger changes in viability than did level changes of residual water in the cells. Although the results are complicated by a large number of interactions, they confirm and extend the hypothesis that reactions between carbonyl compounds and cellular components are a major cause of the mortality occurring during storage of dried microorganisms. A particular mixture of sucrose, glutamate, and semicarbazide is proposed as a means of reducing death when prolonged storage in the dry state is desired.
    • References:
      Appl Microbiol. 1973 Aug;26(2):206-10. (PMID: 4200630)
    • Accession Number:
      0 (Carbon Radioisotopes)
      0 (Glutamates)
      0 (Semicarbazides)
      059QF0KO0R (Water)
      57-50-1 (Sucrose)
      681HV46001 (Ribose)
      K3Z4F929H6 (Lysine)
      OF5P57N2ZX (Alanine)
    • Publication Date:
      Date Created: 19740401 Date Completed: 19740703 Latest Revision: 20210526
    • Publication Date:
      20240829
    • Accession Number:
      PMC380110
    • Accession Number:
      10.1128/am.27.4.648-652.1974
    • Accession Number:
      4207761