H 2 -driven xylitol production in Cupriavidus necator H16.

Publisher: BioMed Central Country of Publication: England NLM ID: 101139812 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2859 (Electronic) Linking ISSN: 14752859 NLM ISO Abbreviation: Microb Cell Fact Subsets: MEDLINE

Original Publication: London : BioMed Central, [2002-

Cupriavidus necator*/metabolism ; Cupriavidus necator*/genetics ; Xylitol*/biosynthesis ; Xylitol*/metabolism ; Hydrogen*/metabolism; Aldehyde Reductase/metabolism ; Aldehyde Reductase/genetics ; Xylose/metabolism ; Biocatalysis

Background: Biocatalysis offers a potentially greener alternative to chemical processes. For biocatalytic systems requiring cofactor recycling, hydrogen emerges as an attractive reducing agent. Hydrogen is attractive because all the electrons can be fully transferred to the product, and it can be efficiently produced from water using renewable electricity. In this article, resting cells of Cupriavidus necator H16 harboring a NAD-dependent hydrogenase were employed for cofactor recycling to reduce D-xylose to xylitol, a commonly used sweetener. To enable this bioconversion, D-xylose reductase from Scheffersomyces stipitis was heterologously expressed in C. necator.
Results: D-xylose reductase was successfully expressed in C. necator, enabling almost complete bioconversion of 30 g/L of D-xylose into xylitol. It was found that over 90% of the energy and protons derived from hydrogen were spent for the bioconversion, demonstrating the efficiency of the system. The highest xylitol productivity reached was 0.7 g/L/h. Additionally, the same chassis efficiently produced L-arabitol and D-ribitol from L-arabinose and D-ribose, respectively.
Conclusions: This study highlights the efficient utilization of renewable hydrogen as a reducing agent to power cofactor recycling. Hydrogen-oxidizing bacteria, such as C. necator, can be promising hosts for performing hydrogen-driven biocatalysis.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)

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342124 Research Council of Finland

Keywords: Cupriavidus necator; Ralstonia eutropha; Biotransformation; Cofactor recycling; Cofactor regeneration; Hydrogen-oxidizing bacteria

VCQ006KQ1E (Xylitol)
7YNJ3PO35Z (Hydrogen)
EC 1.1.1.21 (Aldehyde Reductase)
A1TA934AKO (Xylose)

Date Created: 20241224 Date Completed: 20241224 Latest Revision: 20241224

20241224

10.1186/s12934-024-02615-7

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