Langendorff Isolated Heart Perfusion Model for Functional Studies in Mice.

Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,

Perfusion*/methods ; Isolated Heart Preparation* ; Heart*/physiology; Animals ; Mice

The ex vivo isolated heart perfusion model by Oskar Langendorff to resuscitate and maintain the automacity of a heart excised from a mammal was introduced in the late nineteenth century. It has remained an essential technique used for functional studies in cardiovascular research even after over a hundred years. This model provides information on contractility, cardiac rhythm, metabolism, and overall cardiac function. It allows the study of the heart in isolation without the influences of other organs or hormones. It enables the investigation of physiological mechanisms underlying heart function under different conditions with more experimental control than the heart in vivo. This chapter provides an overview of the protocol for the isolated heart perfusion model.
(© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

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Keywords: Buffer; Cardiac function; Isolated heart; Langendorff; Perfusion

Date Created: 20241219 Date Completed: 20241219 Latest Revision: 20241219

20241219

10.1007/978-1-0716-4342-6_9

39699813