Electrochemical imaging of neurotransmitter release with fast-scan voltammetric ion conductance microscopy.

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  • Additional Information
    • Source:
      Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : American Association for the Advancement of Science, [2015]-
    • Subject Terms:
      Neurotransmitter Agents*/metabolism ; Dopamine*/metabolism ; Astrocytes*/metabolism; Animals ; Electrochemical Techniques/methods ; Synaptic Transmission/physiology ; Microscopy/methods ; Dopaminergic Neurons/metabolism ; Humans ; Rats ; Coculture Techniques
    • Abstract:
      Understanding the dynamic spatial and temporal release of neurotransmitters can help resolve long-standing questions related to chemical modulation of neurological circuits. Dopamine modulates function in a range of physiological processes and is key to transmission in addiction and neurological disorders. Studies at subcellular scales promise to help develop a broader understanding of dopamine release, diffusion, and receptor activation and how these processes lead to functional outcomes. Electrochemical measurements of dopamine release at individual cells have proven especially informative. We describe incorporation of fast-scan cyclic voltammetry for detection of dopamine release with subcellular spatial resolution and millisecond time resolution. The platform is benchmarked with standard redox probes and then applied to imaging stimulated release from subcellular locations of a coculture of dopaminergic neurons and astrocytes. Voltammetry reveals heterogeneity in release based on time, location, and neuron identity. We believe that this platform ultimately offers a window to understanding neurotransmission in pathophysiological models of disease where cell-cell communication is key.
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    • Accession Number:
      0 (Neurotransmitter Agents)
      VTD58H1Z2X (Dopamine)
    • Publication Date:
      Date Created: 20241211 Date Completed: 20241211 Latest Revision: 20241213
    • Publication Date:
      20241213
    • Accession Number:
      PMC11633752
    • Accession Number:
      10.1126/sciadv.ado9322
    • Accession Number:
      39661692