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DNA shuffling to improve crude-water interfacial activity in biosurfactants with OmpA protein of Escherichia coli .
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- Additional Information
- Source:
Publisher: PeerJ Inc Country of Publication: United States NLM ID: 101603425 Publication Model: eCollection Cited Medium: Internet ISSN: 2167-8359 (Electronic) Linking ISSN: 21678359 NLM ISO Abbreviation: PeerJ Subsets: MEDLINE
- Publication Information:
Original Publication: Corte Madera, CA : PeerJ Inc.
- Subject Terms:
- Abstract:
Surfactants are molecules derived primarily from petroleum that can reduce the surface tension at interfaces. Their slow degradation is a characteristic that could cause environmental issues. This and other factors contribute to the allure of biosurfactants today. Progress has been made in this area of research, which aims to satisfy the need for effective surfactants that are not harmful to the environment. In previous studies, we demonstrated the surface tension activity of the Escherichia coli transmembrane protein OmpA. Here, we carried out DNA shuffling on ompA to improve its interfacial activity. We evaluated changes in interfacial tension when exposing mutants to a water-oil interface to identify the most promising candidates. Two mutants reached an interfacial tension value lower (9.10 mN/m and 4.24 mN/m) than the original protein OmpA (14.98 mN/m). Since predicted isoelectric point values are far from neutral pH, the charge of the protein was a crucial factor in explaining the migration of proteins towards the interface. Low molecular weight mutants did not exhibit a significant difference in their migration to the interface.
Competing Interests: The authors declare there are no competing interests.
(©2024 Nuñez Velez et al.)
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- Contributed Indexing:
Keywords: Biosurfactant; DNA shuffling; E. coli; OmpA; Porins
- Accession Number:
149024-69-1 (OMPA outer membrane proteins)
0 (Bacterial Outer Membrane Proteins)
0 (Surface-Active Agents)
059QF0KO0R (Water)
0 (Escherichia coli Proteins)
- Publication Date:
Date Created: 20241209 Date Completed: 20241209 Latest Revision: 20241211
- Publication Date:
20241211
- Accession Number:
PMC11623092
- Accession Number:
10.7717/peerj.17239
- Accession Number:
39650551
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