Protocol for measuring anti-fentanyl antibodies in mouse serum by enzyme-linked immunosorbent assay.

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  • Additional Information
    • Source:
      Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Cambridge, MA] : Cell Press, [2020]-
    • Subject Terms:
      Fentanyl*/immunology ; Fentanyl*/blood ; Enzyme-Linked Immunosorbent Assay*/methods ; Mice, Inbred BALB C* ; Antibodies*/immunology ; Antibodies*/blood ; Mice, Inbred C57BL*; Animals ; Mice
    • Abstract:
      Enzyme-linked immunosorbent assay (ELISA) offers an effective, inexpensive, and reliable approach for the analysis of humoral immune responses. Here, we describe a protocol for measuring anti-fentanyl antibodies generated by the immunization of mice with novel opioid vaccine candidates. We describe steps for coating BSA-fentanyl antigen and standard wells. We then detail procedures for preparing buffers and performing assays. This protocol is adaptable for use with either BALB/c or C57BL/6J mice strains and can measure antibody isotypes to elucidate class switching. For complete details on the use and execution of this protocol, please refer to Stone et al. 1 .
      Competing Interests: Declaration of interests D.S., O.L., and D.J.D. are named inventors on vaccine adjuvant patents assigned to Boston Children’s Hospital. O.L. has served as a paid consultant to GlaxoSmithKline (GSK) and HilleVax. D.J.D. is on the scientific advisory board of EdJen BioTech and serves as a consultant with Merck Research Laboratories/Merck Sharp & Dohme Corp (a subsidiary of Merck & Co., Inc.). D.J.D., O.L., and C.N.H. are cofounders and sit as scientific advisors of Ovax, Inc. E.B.N. is a scientific advisor to Ovax, Inc. These commercial or financial relationships are unrelated to the current study. C.N.H. is named as an inventor on a patent for anti-fentanyl vaccine assigned to the University of Houston.
      (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: antibody; cell biology; immunology
    • Accession Number:
      UF599785JZ (Fentanyl)
      0 (Antibodies)
    • Publication Date:
      Date Created: 20241208 Date Completed: 20241224 Latest Revision: 20241224
    • Publication Date:
      20241224
    • Accession Number:
      10.1016/j.xpro.2024.103402
    • Accession Number:
      39645655