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Bacterial microcompartment utilization in the human commensal Escherichia coli Nissle 1917.
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- Additional Information
- Source:
Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 2985120R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5530 (Electronic) Linking ISSN: 00219193 NLM ISO Abbreviation: J Bacteriol Subsets: MEDLINE
- Publication Information:
Original Publication: Washington, DC : American Society for Microbiology
- Subject Terms:
- Abstract:
Bacterial microcompartments (BMCs) are self-assembled protein structures often utilized by bacteria as a modular metabolic unit, enabling the catalysis and utilization of less common carbon and nitrogen sources within a self-contained compartment. The ethanolamine (EA) utilization (eut) BMC has been widely demonstrated in enteropathogens, such as Salmonella enterica , and current research is exploring its activity in the commensal species that populate the human gut. Escherichia coli Nissle 1917 (EcN) is a strong colonizer and probiotic in gut microbial communities and has been used extensively for microbiome engineering. In this study, the utilization of ethanolamine as a sole carbon source and the formation of the eut BMC in EcN were demonstrated through growth assays and visualization with transmission electron microscopy. Subsequently, flux balance analysis was used to further investigate the metabolic activity of this pathway. It was found that not only is the utilization of the eut BMC for the degradation of EA as a carbon source in EcN comparable with that of Salmonella enterica but also that ammonium is released into solution as a byproduct in EcN but not in S. enterica . Control of EA-dependent growth was demonstrated using different concentrations of the operon inducer, vitamin B 12 . We show that vitamin B 12 -dependent EA utilization as the sole carbon source enables growth in EcN, and demonstrate the concurrent formation of the BMC shell and inducible control of the eut operon.
Importance: The human gut is a complex environment of different bacterial species, nutrient sources, and changing conditions that are essential for human health. An imbalance can allow for the emergence of opportunistic pathogens. Bacterial microcompartments (BMCs) are utilized by bacteria to metabolize less common nutrients, conferring a growth advantage. Although widely studied in enteropathogens, there is limited research on BMC activity in commensal species. We demonstrate the formation of the eut BMC and utilization of ethanolamine as a carbon source in the human gut commensal Escherichia coli Nissle 1917 (EcN). Additionally, we found increased ammonium production when EcN utilized ethanolamine but did not see the same in Salmonella enterica , highlighting potential differences in how these species affect the wider microbial community.
Competing Interests: The authors declare no conflict of interest.
- Grant Information:
BB/T008709/1 UKRI | Biotechnology and Biological Sciences Research Council (BBSRC); EP/W004674/1 UKRI | Engineering and Physical Sciences Research Council (EPSRC); EP/R013756/1 UKRI | Engineering and Physical Sciences Research Council (EPSRC); 770835 EC | European Research Council (ERC); 209409/Z/17/Z Wellcome Trust (WT)
- Contributed Indexing:
Keywords: E. coli Nissle 1917; bacterial microcompartment; ethanolamine catabolism; microbiome
- Accession Number:
5KV86114PT (Ethanolamine)
7440-44-0 (Carbon)
0 (Escherichia coli Proteins)
- Publication Date:
Date Created: 20241205 Date Completed: 20241219 Latest Revision: 20241219
- Publication Date:
20241219
- Accession Number:
10.1128/jb.00269-24
- Accession Number:
39636254
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