Identification of essential genes for Acanthamoeba castellanii excystation during encystation and excystation.

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  • Additional Information
    • Source:
      Publisher: The Korean Society for Parasitology and Tropical Medicine Country of Publication: Korea (South) NLM ID: 9918574074806676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2982-6799 (Electronic) Linking ISSN: 29825164 NLM ISO Abbreviation: Parasites Hosts Dis Subsets: MEDLINE
    • Publication Information:
      Original Publication: Sŏul : The Korean Society for Parasitology and Tropical Medicine, February 2023-
    • Subject Terms:
      Acanthamoeba castellanii*/genetics ; Acanthamoeba castellanii*/growth & development; Genes, Essential/genetics ; Trophozoites ; Gene Expression Profiling ; Parasite Encystment/genetics ; Life Cycle Stages/genetics ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism ; Genes, Protozoan/genetics
    • Abstract:
      Acanthamoeba is an opportunistic pathogen that causes Acanthamoeba keratitis, granulomatous amoebic encephalitis, and other cutaneous diseases. The life cycle of Acanthamoeba consists of 2 stages of trophozoites and cysts. Under adverse environmental conditions, Acanthamoeba encysts, while the conditions become favorable for growth, it reverts to the trophozoite form. Acanthamoeba excystation is crucial for its proliferation and can lead to recurrent infections after incomplete treatment. To identify the factors involved in excystation, A. castellanii was subjected to either encystation- or excystation-inducing conditions, and gene expression profiles were compared using mRNA sequencing. A. castellanii samples were collected at 8 h intervals for analysis under both conditions. Differentially expressed gene analysis revealed that 1,214 and 1,163 genes were upregulated and downregulated, respectively, by more than 2-fold during early excystation. Five genes markedly upregulated in early excystation (ACA1_031140, ACA1_032330, ACA1_374400, ACA1_275740, and ACA1_112650) were selected, and their expression levels were confirmed via real-time PCR. Small interfering RNA (siRNA) targeting these 5 genes was transfected into Acanthamoeba and gene knockdown was validated through real-time PCR. The silencing of ACA1_031140, ACA1_032330, ACA1_374400, and ACA1_112650 inhibited excystation and suggested that these genes might be essential for excystation. Our findings provide valuable insights for suppressing Acanthamoeba proliferation and recurrence.
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    • Grant Information:
      RS-2024-00346635 National Research Foundation of Korea; Ministry of Science and ICT
    • Contributed Indexing:
      Keywords: Acanthamoeba; encystation; excystation; gene knockdown
    • Accession Number:
      0 (Protozoan Proteins)
    • Publication Date:
      Date Created: 20241202 Date Completed: 20241202 Latest Revision: 20241205
    • Publication Date:
      20241209
    • Accession Number:
      PMC11614486
    • Accession Number:
      10.3347/PHD.24062
    • Accession Number:
      39622652