Jianpi Huoxue Decoction Ameliorates Alcohol-associated Liver Disease by Improving Intestinal Barrier Function in Rats.

Publisher: Huazhong University of Science and Technology Country of Publication: China NLM ID: 101729993 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2523-899X (Electronic) Linking ISSN: 2523899X NLM ISO Abbreviation: Curr Med Sci Subsets: MEDLINE

Original Publication: Wuhan : Huazhong University of Science and Technology, [2018]-

Drugs, Chinese Herbal*/pharmacology ; Liver Diseases, Alcoholic*/drug therapy ; Liver Diseases, Alcoholic*/metabolism ; Liver Diseases, Alcoholic*/pathology ; Rats, Sprague-Dawley* ; Intestinal Mucosa*/drug effects ; Intestinal Mucosa*/metabolism ; Intestinal Mucosa*/pathology; Animals ; Rats ; Male ; Tight Junctions/drug effects ; Tight Junctions/metabolism ; Disease Models, Animal ; Zonula Occludens-1 Protein/metabolism ; Zonula Occludens-1 Protein/genetics ; Intestines/drug effects ; Intestines/pathology ; Lipopolysaccharides ; Occludin/metabolism ; Occludin/genetics ; Intestinal Barrier Function

Objective: Jianpi huoxue decoction (JHD), a Chinese herbal formula, is commonly used for treating alcohol-associated liver disease (ALD). This study aimed to investigate the mechanism by which JHD affects intestinal barrier function in ALD rats.
Methods: The Sprague-Dawley rats were randomly divided into three groups: control group, model group and JHD group. They were pair-fed a modified Lieber-DeCarli liquid diet containing alcohol (model group, n=10; JHD group, n=10) or isocaloric maltose dextrin (control group, n=10) for 6 weeks. After 3 weeks of feeding, the mice in the JHD group were given JHD (10 mL/kg/day) by gavage for 3 weeks, and those in the control and model groups received equal amounts of double-distilled water for the same period of time. Afterwards, all the rats were given lipopolysaccharide (LPS) by gavage and sacrificed 3.5 h later. LPS levels were measured in the portal blood to evaluate gut leakage. Transmission electron microscopy (TEM) was used to observe ultrastructural changes in the intestinal tract. Adherens junction (AJ) and tight junction (TJ) proteins were detected by Western blotting, immunofluorescence or immunohistochemistry.
Results: JHD ameliorated Lieber-DeCarli liquid diet-induced hepatic steatosis, inflammation and LPS expression. It improved pathological changes in the liver and alleviated intestinal ultrastructure injury. Moreover, it significantly enhanced the integrity of tight junctions by increasing the expression of zonula occludens-1 (ZO-1) and occludin. It suppressed the activation of myosin light chain (MLC) phosphorylation.
Conclusion: JHD improves intestinal barrier function and reduces gut leakiness in ALD rats.
Competing Interests: Conflict of Interest Statement. The authors declare that there is no conflict of interest with any financial organization or corporation or individual that can inappropriately influence this work.
(© 2024. Huazhong University of Science and Technology.)

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Keywords: Jianpi Huoxue decoction; alcohol-associated liver disease; intestinal barrier function; intestinal permeability; lipopolysaccharide; tight junction

0 (Drugs, Chinese Herbal)
0 (Jianpi Huoxue decoction)
0 (Zonula Occludens-1 Protein)
0 (Lipopolysaccharides)
0 (Occludin)

Date Created: 20241201 Date Completed: 20241223 Latest Revision: 20241223

20241223

10.1007/s11596-024-2955-2

39617866