Renshen (Radix Ginseng) polysaccharide promotes repair of the mice intestinal mucosa through regulatory mechanisms based on polyamine and human antigen R.

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  • Additional Information
    • Source:
      Publisher: Academy Of Traditional Chinese Medicine Country of Publication: China NLM ID: 8211546 Publication Model: Print Cited Medium: Internet ISSN: 2589-451X (Electronic) Linking ISSN: 02552922 NLM ISO Abbreviation: J Tradit Chin Med Subsets: MEDLINE
    • Publication Information:
      Publication: Beijing : Academy Of Traditional Chinese Medicine
      Original Publication: [Beijing, China : Pub. Office, Journal of Traditional Chinese Medicine, 1981-
    • Subject Terms:
      Panax*/chemistry ; Intestinal Mucosa*/drug effects ; Intestinal Mucosa*/metabolism ; Polysaccharides*/pharmacology ; Cell Movement*/drug effects; Animals ; Mice ; Humans ; Male ; Drugs, Chinese Herbal/administration & dosage ; Drugs, Chinese Herbal/pharmacology ; ELAV-Like Protein 1/metabolism ; ELAV-Like Protein 1/genetics ; cdc42 GTP-Binding Protein/metabolism ; cdc42 GTP-Binding Protein/genetics ; Cell Line ; Mice, Inbred C57BL ; Rats
    • Abstract:
      Objective: To investigate the mechanism and effect of Renshen ( Radix Ginseng ) polysaccharide on the migration of intestinal epithelial cell line 6 (IEC-6), as well as the repair mechanism of Renshen ( Radix Ginseng ) polysaccharide on colonic injury induced by dextran sulfate sodium (DSS) in mice.
      Methods: Mice were fed 3% (w/v) DSS for 6 d to create colonic lesions. A cell-migration model was created using cell scratching. mRNA expression, protein expression, translation efficiency of mRNA, and nucleoplasmic distribution of human antigen R (HuR) were determined by real-time reverse transcription-quantitative polymerase chain reaction, western blotting, a dual luciferase reporter system, and immunofluorescence staining, respectively.
      Results: Renshen ( Radix Ginseng ) polysaccharide promoted the migration of IEC-6 cells and affected expression of stromal interaction molecule 1 (STIM1) and cell division cycle 42 (Cdc42) at transcriptional and post-transcriptional levels.
      Conclusions: Renshen ( Radix Ginseng ) polysaccharide-induced repair of intestinal mucosal injury may be mediated by increased cell migration via polyamine-based regulatory mechanisms. In vitro and in vivo experiments suggest that Renshen ( Radix Ginseng ) polysaccharide-induced post-transcriptional regulation of STIM1 and Cdc42 may be related to differences in the regulation of different target genes by HuR. Taken together, these data provide a reference for further exploration of the protective effect of Renshen ( Radix Ginseng ) on the intestinal mucosa.
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    • Grant Information:
      81673940 National Natural Science Foundation of China: Study on the Effect of Supplementing Qi and Invigorating the Spleen on the Migration of Small Intestinal Epithelial Cells through the Regulation of Polyamines and Calcium Ions; 62 First-class Discipline Construction Major Project of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine Planning (2020) No. 62: based on the Viscous Characteristics of Dampness and Pathogenic Factors in Lingnan, this Paper Studied the Differential Characteristics of the Syndrome of Deficiency of Spleen and Stomach Disease and the Intervention Mechanism of Lingnan Traditional Chinese Medicine for Mucosal Damage Repair
    • Contributed Indexing:
      Keywords: Renshen (); cell movement; human antigen R; intestinal mucosal damage repair; polysaccharides; post-transcriptional regulation
    • Accession Number:
      0 (Polysaccharides)
      0 (Drugs, Chinese Herbal)
      0 (ELAV-Like Protein 1)
      EC 3.6.5.2 (cdc42 GTP-Binding Protein)
    • Publication Date:
      Date Created: 20241201 Date Completed: 20241202 Latest Revision: 20241201
    • Publication Date:
      20241204
    • Accession Number:
      PMC11589556
    • Accession Number:
      10.19852/j.cnki.jtcm.2024.06.003
    • Accession Number:
      39617697