Decelerated skin aging effect of rubber (Hevea brasiliensis) seed oil in cell culture assays.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • Subject Terms:
      Skin Aging*/drug effects ; Hevea*/chemistry ; Plant Oils*/pharmacology ; Plant Oils*/chemistry ; Seeds*/chemistry ; Keratinocytes*/drug effects ; Keratinocytes*/metabolism; Humans ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Cell Proliferation/drug effects ; Fatty Acids/analysis ; Fatty Acids/metabolism ; Matrix Metalloproteinase 2/metabolism ; Gas Chromatography-Mass Spectrometry ; Antioxidants/pharmacology ; Cell Line
    • Abstract:
      Rubber seeds, the abundant by-products of rubber tree (Hevea brasiliensis), have been studied for sustainable utilization. Nevertheless, there is no information available regarding activity against skin aging. The study aimed to prepare rubber seed oil (RSO) and evaluate fatty acid compositions by gas chromatography - mass spectrometry (GC/MS), linamarin contamination by ultra-high performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS). Additionally, cytotoxicity assay and anti-skin aging activities, including cell proliferating stimulation, cellular antioxidant, collagen stimulation, and matrix metalloproteinase-2 (MMP-2) inhibition, were analyzed in immortalized human skin keratinocytes (HaCaT cells) and human dermal fibroblasts. RSO was pale-yellow oily liquid with an extraction yield of 35.79 ± 0.52%. Principal fatty acids were comprised of oleic (43.37 ± 0.76%), linoleic (38.49 ± 0.81%), palmitic (11.47 ± 0.12%), and stearic (6.66 ± 0.05%) acids. Linamarin contamination was not detected in 100 µg/mL RSO, demonstrating the absence of a cyanogenic glucoside. Non-cytotoxic concentrations of RSO in both cells were in the range of 0.0001-0.1 mg/mL. Activities of RSO against skin aging included the cell proliferating stimulation, the antioxidant activity, the collagen stimulation, and the MMP-2 suppression at mRNA expression level and enzymatic activity. Study results have suggested that rubber seeds can probably be employed as a promising ingredient in the preparations designed for deceleration of skin aging.
      Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
      (© 2024. The Author(s).)
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    • Grant Information:
      662A02016 National Science, Research and Innovation Fund (NSRF)
    • Contributed Indexing:
      Keywords: Antioxidant activity; Collagen stimulation; Matrix metalloproteinase-2 suppression; Rubber seeds; Skin aging
    • Accession Number:
      0 (Plant Oils)
      0 (Fatty Acids)
      EC 3.4.24.24 (Matrix Metalloproteinase 2)
      0 (Antioxidants)
    • Publication Date:
      Date Created: 20241128 Date Completed: 20241128 Latest Revision: 20241202
    • Publication Date:
      20241204
    • Accession Number:
      PMC11603368
    • Accession Number:
      10.1038/s41598-024-81035-4
    • Accession Number:
      39604582