Effectiveness of Local Use of Green Propolis-Loaded Lipid Nanoparticles as Adjuvant Therapy to Scaling and Root Planing in the Management of Periodontitis in Rats Treated with Zoledronate.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • Subject Terms:
      Zoledronic Acid* ; Periodontitis*/therapy ; Periodontitis*/drug therapy ; Propolis*/pharmacology ; Nanoparticles*/chemistry ; Root Planing*/methods; Animals ; Female ; Rats ; Dental Scaling/methods ; Liposomes/chemistry ; Rats, Wistar ; Tumor Necrosis Factor-alpha/metabolism ; Interleukin-1beta/metabolism ; Ovariectomy
    • Abstract:
      This study assessed the effectiveness of the local use of green propolis-loaded lipid nanoparticles (GPlnp) as an adjuvant therapy to scaling and root planing (SRP) to manage experimental periodontitis (EP) in ovariectomized rats treated with zoledronate. Ten weeks before the experiment, 48 female rats were ovariectomized. On day 0, a ligature was installed in the lower first molar to induce EP. From day 0 to day 42, half of the rats were treated with vehicle (VEH), while the other half were treated with 100μg/Kg of zoledronate (ZOL). On day 14, the rats were allocated into the following groups: VEH-NLT, VEH-SRP, VEH-SRP-GPlnp, ZOL-NLT, ZOL-SRP, and ZOL-SRP-GPlnp. VEH-NLT and ZOL-NLT received no local treatment. VEH-SRP and ZOL-SRP received SRP and irrigation with physiological saline solution. VEH-SRP-GPlnp and ZOL-SRP-GPlnp received SRP and irrigation with GPlnp. A single SRP session was carried out, and four irrigation sessions were conducted (on days 14, 16, 18, and 20). On day 42, all animals were euthanized. The hemimandibles were processed for histological, histometric (percentage of total bone tissue (PTBT) and non-vital bone tissue (PNVBT)) and immunohistochemical (TNFα, IL-1β, and TRAP) analysis. VEH-SRP-GPlnp showed better tissue repair, higher PTBT, and lower immunolabeling for TNFα and IL-1β compared to the groups treated with VEH. ZOL-SRP-GPlnp showed a favorable tissue repair, with lower PNVBT, less local inflammation, and lower immunolabeling for TNFα and IL-1β compared to the groups treated with ZOL. Irrigation with GPlnp proved to be effective as an adjuvant therapy to SRP in treating EP in ovariectomized rats treated with zoledronate.
    • References:
      Molecules. 2022 Nov 22;27(23):. (PMID: 36500223)
      Molecules. 2024 Jun 18;29(12):. (PMID: 38930964)
      Front Physiol. 2023 Jul 12;14:1210449. (PMID: 37501927)
      Drug Des Devel Ther. 2024 May 01;18:1469-1495. (PMID: 38707615)
      Fitoterapia. 2024 Jun;175:105975. (PMID: 38685509)
      J Oral Maxillofac Surg. 2022 May;80(5):920-943. (PMID: 35300956)
      Phytother Res. 2021 May;35(5):2274-2286. (PMID: 32935428)
      J Periodontal Implant Sci. 2022 Feb;52(1):39-53. (PMID: 35187872)
      J Periodontol. 2008 Jun;79(6):1089-94. (PMID: 18533788)
      J Ethnopharmacol. 2021 Oct 5;278:114255. (PMID: 34062248)
      J Clin Periodontol. 2020 Jul;47 Suppl 22:155-175. (PMID: 31889320)
      Cochrane Database Syst Rev. 2022 Jul 12;7:CD012432. (PMID: 35866376)
      Clin Oral Investig. 2022 May;26(5):3843-3852. (PMID: 35482084)
      J Clin Med. 2023 Jun 27;12(13):. (PMID: 37445360)
      Arch Oral Biol. 2024 Mar;159:105875. (PMID: 38160519)
      Saudi Dent J. 2022 Mar;34(3):202-210. (PMID: 35935720)
      Photodiagnosis Photodyn Ther. 2022 Dec;40:103172. (PMID: 36283616)
      Photochem Photobiol. 2021 Sep;97(5):1104-1115. (PMID: 33713346)
      J Food Biochem. 2022 Oct;46(10):e14350. (PMID: 35880944)
      J Periodontol. 2014 Feb;85(2):226-33. (PMID: 23786404)
      Int J Periodontics Restorative Dent. 2006 Aug;26(4):315-9. (PMID: 16939012)
      J Oral Pathol Med. 2014 May;43(5):371-7. (PMID: 24450511)
      J Dent Res. 2018 Jul;97(8):928-936. (PMID: 29494308)
      Nanomaterials (Basel). 2020 Aug 24;10(9):. (PMID: 32846957)
      Endocrinology. 2012 Jan;153(1):17-28. (PMID: 22109892)
      Cureus. 2024 Apr 28;16(4):e59234. (PMID: 38813303)
      AMB Express. 2024 Jul 16;14(1):81. (PMID: 39014110)
      J Bone Miner Res. 2011 Aug;26(8):1871-82. (PMID: 21351151)
      J Nanobiotechnology. 2024 Jan 4;22(1):19. (PMID: 38178140)
      Support Care Cancer. 2015 Jul;23(7):2007-17. (PMID: 25519757)
      J Bone Miner Res. 2022 Jul;37(7):1400-1410. (PMID: 35598324)
      Evid Based Complement Alternat Med. 2022 Jul 21;2022:5798941. (PMID: 35911156)
      Endocrinol Metab (Seoul). 2021 Oct;36(5):917-927. (PMID: 34674506)
      Front Cell Infect Microbiol. 2023 Mar 28;13:1104826. (PMID: 37056703)
      Metabolites. 2024 Jul 06;14(7):. (PMID: 39057701)
      Dent Clin North Am. 2024 Jan;68(1):67-85. (PMID: 37951638)
      PLoS One. 2024 Jul 16;19(7):e0307289. (PMID: 39012879)
      J Oral Biosci. 2021 Mar;63(1):58-65. (PMID: 33484883)
      Sci Rep. 2019 Jan 10;9(1):22. (PMID: 30631095)
      Phytother Res. 2016 Jun;30(6):894-905. (PMID: 26988443)
      J Adv Periodontol Implant Dent. 2021 Sep 26;13(2):84-89. (PMID: 35919679)
      Saudi J Biol Sci. 2019 Nov;26(7):1695-1703. (PMID: 31762646)
      Arch Razi Inst. 2022 Oct 31;77(5):1673-1681. (PMID: 37123150)
      Br J Oral Maxillofac Surg. 2020 Jul;58(6):619-624. (PMID: 32247520)
      Dent J (Basel). 2019 May 01;7(2):. (PMID: 31052358)
      Odontology. 2020 Oct;108(4):704-714. (PMID: 32112365)
      Sci Rep. 2024 Jun 13;14(1):13613. (PMID: 38871855)
      Lett Appl Microbiol. 2016 Feb;62(2):192-8. (PMID: 26643709)
      J Clin Periodontol. 2018 May;45(5):570-577. (PMID: 29479739)
      Pharmaceuticals (Basel). 2021 May 12;14(5):. (PMID: 34065862)
      Front Oral Biol. 2012;15:117-32. (PMID: 22142960)
      Molecules. 2019 May 29;24(11):. (PMID: 31146392)
      J Appl Microbiol. 2017 Apr;122(4):911-920. (PMID: 28066967)
      Bone. 2018 Mar;108:79-88. (PMID: 29289789)
      Bone. 2019 Mar;120:101-113. (PMID: 30339908)
      Sci Rep. 2024 Jan 5;14(1):627. (PMID: 38182598)
      Microorganisms. 2022 Oct 31;10(11):. (PMID: 36363764)
      Oral Dis. 2018 May;24(4):527-536. (PMID: 28656643)
      Int J Mol Sci. 2022 Nov 10;23(22):. (PMID: 36430343)
      Colloids Surf B Biointerfaces. 2021 Apr;200:111571. (PMID: 33476953)
      JBMR Plus. 2023 Jun 22;7(8):e10785. (PMID: 37614299)
      Support Care Cancer. 2022 Mar;30(3):1967-1980. (PMID: 34633539)
      J Vet Dent. 2024 Jan;41(1):49-57. (PMID: 37259505)
      Bone. 2021 Dec;153:116168. (PMID: 34487892)
      J Oral Pathol Med. 2020 Mar;49(3):190-200. (PMID: 31605632)
      J Periodontol. 2021 Oct;92(10):1370-1378. (PMID: 33660307)
    • Grant Information:
      #2019/17769-1; #30699-2014 São Paulo Research Foundation (FAPESP), National Council for Scientific and Technological Development (CNPq), and Coordination of Superior Level Staff Improvement (CAPES).
    • Contributed Indexing:
      Keywords: nanoparticles; periodontitis; propolis; scaling and root planing; zoledronate
    • Accession Number:
      6XC1PAD3KF (Zoledronic Acid)
      9009-62-5 (Propolis)
      0 (Lipid Nanoparticles)
      0 (Liposomes)
      0 (Tumor Necrosis Factor-alpha)
      0 (Interleukin-1beta)
    • Publication Date:
      Date Created: 20241127 Date Completed: 20241127 Latest Revision: 20241130
    • Publication Date:
      20241202
    • Accession Number:
      PMC11595208
    • Accession Number:
      10.3390/ijms252212443
    • Accession Number:
      39596508