Genome-Wide Analysis of HECT E3 Ligases Members in Phyllostachys edulis Provides Insights into the Role of PeHECT1 in Plant Abiotic Stress Response.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Ubiquitin-Protein Ligases*/genetics ; Ubiquitin-Protein Ligases*/metabolism ; Gene Expression Regulation, Plant* ; Stress, Physiological*/genetics ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Phylogeny*; Promoter Regions, Genetic/genetics ; Genome, Plant ; Genome-Wide Association Study ; Nicotiana/genetics ; Plant Growth Regulators/metabolism

Homology to E6-AP Carboxy Terminus (HECT) E3 ubiquitin ligases play pivotal roles in plant growth, development, and responses to abiotic stresses. However, the function of HECT genes in Phyllostachys edulis ( P. edulis ) remains largely uninvestigated. In this study, a comprehensive genome-wide analysis of the HECT E3 ubiquitin ligases gene family in P. edulis was conducted, aiming to elucidate its evolutionary relationships and gene expansion. Analysis of gene structure, conserved motifs and domains, and synteny genome regions were performed. Furthermore, cis-elements in HECT gene promoters that respond to plant hormones and environmental stresses were identified and corroborated by expression data from diverse abiotic stress conditions and hormone treatments. Based on the co-expression network of PeHECTs under cold and dehydration stresses, PeHECT1 was identified as a key candidate gene associated with abiotic stress tolerance. Overexpression of PeHECT1 in tobacco leaves significantly upregulated genes related to reactive oxygen species (ROS) detoxification and polyamine biosynthesis. Yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and dual-luciferase (dual-LUC) assays suggested that the transcription factor ETHYLENE RESPONSE FACTOR 3 (PeERF3) bound to the dehydration-responsive element (DRE) of the promoter of PeHECT1 and activated its transcription activity. Phylogenetic analysis indicated that PeHECT1 in P. edulis exhibited a close association with the diploid herbaceous bamboo Olyra latifolia , followed by the divergence of rice and bamboo. In summary, this study enhances our comprehensive understanding of the HECT E3 ubiquitin ligases gene family in P. edulis and highlights the potential role of PeHECT1 in plant abiotic stress response.

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CAFYBB2022SY009；CAFYBB2020ZB004 Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry; 2021YFD2200504 National Key Research and Development Program of China; 20242BAB26096 Key Project of Natural Science Foundation of Jiangxi Province in 2024

Keywords: HECT E3 ubiquitin ligases; abiotic stresses; gene family; moso bamboo (Phyllostachys edulis)

EC 2.3.2.27 (Ubiquitin-Protein Ligases)
0 (Plant Proteins)
0 (Plant Growth Regulators)

Date Created: 20241127 Date Completed: 20241127 Latest Revision: 20241130

20241202

PMC11593785

10.3390/ijms252211896

39595966