Re-sensitization of imipenem-resistant Pseudomonas aeruginosa and restoration of cephalosporins susceptibility in Enterobacteriaceae by recombinant Esterase B.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 8510094 Publication Model: Print Cited Medium: Internet ISSN: 1472-765X (Electronic) Linking ISSN: 02668254 NLM ISO Abbreviation: Lett Appl Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2023- : Oxford : Oxford University Press
      Original Publication: Oxford, UK : Published for the Society for Applied Bacteriology by Blackwell Scientific Publications, [c1985-
    • Subject Terms:
      Pseudomonas aeruginosa*/genetics ; Pseudomonas aeruginosa*/drug effects ; Pseudomonas aeruginosa*/enzymology ; Anti-Bacterial Agents*/pharmacology ; Cephalosporins*/pharmacology ; Microbial Sensitivity Tests* ; Imipenem*/pharmacology; Enterobacteriaceae/genetics ; Enterobacteriaceae/drug effects ; Enterobacteriaceae/enzymology ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Esterases/genetics ; Esterases/metabolism ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Drug Resistance, Bacterial ; Escherichia coli/genetics ; Escherichia coli/drug effects ; Plasmids/genetics
    • Abstract:
      Sphingobium sp. SM42 Esterase B (EstB) is an enzyme with a dual function in degrading dibutyl phthalate and catalyzing the cleavage of the C-S bond in C3-sidechains of the dihydrothiazine ring of cephalosporins, generating more active β-lactam derivatives. Global prokaryotic genome analysis revealed the existence of a gene identical to estB in Pseudomonas aeruginosa strain PS1 suggesting a horizontal gene transfer event involving estB. To investigate the effect of ectopic expression of EstB in the periplasm of P. aeruginosa and several Enterobacteriaceae on antibiotic susceptibility levels, plasmid, pEstB, carrying a recombinant EstB fused with the signal peptide from Escherichia coli outer membrane protein A (OmpA) for periplasmic localization was constructed. The expression of EstB in the periplasm of P. aeruginosa and the Enterobacteriaceae: E. coli, Klebsiella pneumoniae, and Salmonella enterica serovar Typhi, increased susceptibility to carbapenems and cephalosporins. EstB reversed the imipenem resistance of P. aeruginosa ΔmexS and restored the changes in susceptibility to cephalosporins conferred by the downregulation of the outer membrane proteins, OmpK35 and OmpK36, in K. pneumoniae ΔramR-ompK36 to wild-type level. The introduction of EstB to the periplasmic space of Gram-negative bacteria can increase carbapenem and cephalosporin susceptibility.
      (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
    • Grant Information:
      Thailand Science Research and Innovation; 49892/4759806 Chulabhorn Research Institute
    • Contributed Indexing:
      Keywords: Pseudomonas aeruginosa; antimicrobial resistance; enterobacteriaceae; esterase B
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Cephalosporins)
      71OTZ9ZE0A (Imipenem)
      0 (Bacterial Proteins)
      EC 3.1.- (Esterases)
      0 (Bacterial Outer Membrane Proteins)
      0 (Recombinant Proteins)
    • Publication Date:
      Date Created: 20241122 Date Completed: 20241210 Latest Revision: 20241210
    • Publication Date:
      20241211
    • Accession Number:
      10.1093/lambio/ovae118
    • Accession Number:
      39577842