Impacts of adding sucrose or trehalose to extenders with different glycerol concentrations on freezablility and fertility of buffalo bull semen.

Publisher: Springer Country of Publication: Switzerland NLM ID: 8100520 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-7446 (Electronic) Linking ISSN: 01657380 NLM ISO Abbreviation: Vet Res Commun Subsets: MEDLINE

Publication: [Cham] : Springer
Original Publication: [Amsterdam] : Elsevier, 1980-

Buffaloes*/physiology ; Trehalose*/pharmacology ; Cryoprotective Agents*/pharmacology ; Semen Preservation*/veterinary ; Semen Preservation*/methods ; Glycerol*/pharmacology ; Cryopreservation*/veterinary ; Cryopreservation*/methods ; Sucrose*/pharmacology; Animals ; Male ; Fertility/drug effects ; Female ; Semen/drug effects ; Semen Analysis/veterinary ; Pregnancy ; Spermatozoa/drug effects ; Spermatozoa/physiology

This experiment was conducted to determine the most suitable glycerol concentration (3 or 6%) and/or non-penetrating cryoprotectants (trehalose and sucrose) for the cryopreservation of buffalo semen, with the aim of enhancing the cryopreservation protocol. Semen of Egyptian buffalo were pooled and diluted with eight Tris extenders supplemented with either 6% glycerol (control group, GL6), 3% (low level, GL3), sucrose (SU, 50 mM), trehalose (TR, 50 mM), 6% glycerol together with 50 mM of sucrose (GL6SU) or 50 mM of trehalose (GL6TR), and 3% of glycerol together with 50 mM of sucrose (GL3SU) or 50 mM of trehalose (GL3TR), then frozen following the standard protocol. Findings indicated that GL3 extender resulted in the highest values of progressive motility, sperm kinematics, sperm membrane integrity, and viability of post-thawed semen (37 °C for 30 s). On the contrary, the Tris extender enriched only with SU and TR groups had the lowest values of sperm quality compared to the other groups (p < 0.05). All GL supplemented groups showed higher intact acrosome levels and lower detached acrosome and dead sperm with intact acrosome compared to those with TR and SU alone (p < 0.05). A significant increase in viable sperm was observed in the GL3, GL6, and GL3SU groups compared to the other groups (p < 0.05). The Tris extender supplemented with low glycerol (3%) significantly reduced the levels of MDA. In the in vivo fertility trial, it was shown that the pregnancy rate was higher in the GL6SU group (72%) than in the GL3SU group (68%; p > 0.05). Collectively, these results suggest that there is potential in using low glycerol (3%) as a cryoprotective agent in the medium for buffalo sperm cryopreservation without significant adverse effects compared to the addition of 6% glycerol. This study supported the sustainability of materials used in assisted reproductive technology by reducing the glycerol content in the freezing medium. Further research is needed to confirm this hypothesis.
Competing Interests: Declarations. Ethics approval and consent to participate: All investigational techniques applied in this research were approved by the Animal Ethics Committee of Zagazig University, based on IACUC (Institutional Animal Use and Care Committee) rules (Approval No: ZU IACUC/2/F/173/2022). The study complies with the European Directive 2010/63/EU regarding husbandry and experimental handling Consent for publication: Not applicable Competing interests: The authors declare no conflict of interest.
(© 2024. The Author(s).)

Biol Reprod. 1993 Jul;49(1):112-23. (PMID: 8353176)
Theriogenology. 2016 Mar 15;85(5):954-959. (PMID: 26653954)
Cryobiology. 1987 Aug;24(4):324-31. (PMID: 3621976)
Animals (Basel). 2023 Sep 20;13(18):. (PMID: 37760374)
Theriogenology. 2009 Mar 15;71(5):872-6. (PMID: 19091389)
Arch Androl. 2007 Mar-Apr;53(2):99-103. (PMID: 17453690)
Reprod Biol Endocrinol. 2018 Oct 19;16(1):102. (PMID: 30340492)
Theriogenology. 2011 Sep 15;76(5):833-42. (PMID: 21664674)
Reprod Domest Anim. 2012 Feb;47(1):143-50. (PMID: 21676035)
Animals (Basel). 2022 Nov 24;12(23):. (PMID: 36496792)
Animal. 2023 May;17 Suppl 1:100764. (PMID: 37567675)
Poult Sci. 2019 Sep 1;98(9):4161-4171. (PMID: 31065720)
Anim Reprod Sci. 2011 Feb;123(3-4):157-62. (PMID: 21288669)
Front Vet Sci. 2019 Aug 27;6:268. (PMID: 31552277)
Sci Rep. 2023 Jun 30;13(1):10621. (PMID: 37391447)
Andrologia. 2018 Feb;50(1):. (PMID: 28224657)
Cryo Letters. 2017 May/Jun;38(3):239-249. (PMID: 28767747)
Fertil Steril. 2009 Apr;91(4):1285-92. (PMID: 18371961)
Vet Res Commun. 2021 Sep;45(2-3):47-74. (PMID: 33929679)
J Adv Vet Anim Res. 2022 Sep 30;9(3):396-404. (PMID: 36382032)
Methods Mol Biol. 2013;927:13-9. (PMID: 22992899)
Theriogenology. 2019 Mar 1;126:121-127. (PMID: 30551018)
Biopreserv Biobank. 2020 Oct;18(5):367-375. (PMID: 32609000)
Theriogenology. 2007 Sep 15;68(5):813-9. (PMID: 17658594)
Anim Reprod Sci. 2023 Nov;258:107330. (PMID: 37734123)
Cytotechnology. 2016 Dec;68(6):2335-2344. (PMID: 27761771)
Cryobiology. 2009 Jun;58(3):287-92. (PMID: 19233153)
Cryobiology. 2020 Feb 1;92:62-66. (PMID: 31669434)
Physiology (Bethesda). 2015 Nov;30(6):438-48. (PMID: 26525343)
Anim Reprod Sci. 2024 May;264:107472. (PMID: 38598888)
Anim Reprod Sci. 2024 Apr;263:107429. (PMID: 38382197)
Theriogenology. 2023 Jul 1;204:31-39. (PMID: 37040685)
Front Physiol. 2021 Nov 25;12:749735. (PMID: 34899383)
Theriogenology. 2012 Apr 15;77(7):1280-9. (PMID: 22326587)
Theriogenology. 1994 Aug 1;42(2):351-60. (PMID: 16727543)

Keywords: Buffalo semen; Low glycerol; Sperm quality, Cryopreservation

B8WCK70T7I (Trehalose)
0 (Cryoprotective Agents)
PDC6A3C0OX (Glycerol)
57-50-1 (Sucrose)

Date Created: 20241120 Date Completed: 20241120 Latest Revision: 20241123

20241123

PMC11579177

10.1007/s11259-024-10573-z

39565475