Comprehensive Analysis of the Gene Expression Profiles of Rat Brain Tissues under Environmental Exposure to Nicotine.

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  • Author(s): Liu W; Lv H; Zhou Y; Zuo X; Wang X
  • Source:
    Pakistan journal of biological sciences : PJBS [Pak J Biol Sci] 2024 Oct; Vol. 27 (11), pp. 547-566.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Asian Network for Scientific Information Country of Publication: Pakistan NLM ID: 101247723 Publication Model: Print Cited Medium: Internet ISSN: 1812-5735 (Electronic) Linking ISSN: 10288880 NLM ISO Abbreviation: Pak J Biol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Faisalabad : Asian Network for Scientific Information
    • Subject Terms:
      Nicotine* ; Brain*/metabolism ; Brain*/drug effects ; Gene Expression Profiling* ; Transcriptome*/drug effects ; Protein Interaction Maps* ; Environmental Exposure*/adverse effects; Animals ; Rats ; Computational Biology/methods ; Gene Regulatory Networks ; Signal Transduction/drug effects
    • Abstract:
      Background and Objective: Nicotine-relevant smoking causes many serious issues of environmental pollution and complicated harm to human health. The present study aimed to evaluate the experimental effects of exposure to nicotine on the gene expression profiles of rat brain tissues with differentially expressed genes (DEGs). Materials and Methods: The rat gene expression profiles of environmental exposure to nicotine were initially screened and retrieved from the microarray dataset GSE59895 in the GEO database. Next, it was analyzed with an integrated bioinformatics pipeline. The DEGs were analyzed in Limma and functional enrichment analyses of GO terms and KEGG pathways were performed with clusterProfiler. The STRING online tools and Cytoscape StringApp were subsequently employed to construct the protein-protein interaction (PPI) network, whereas key modules and hub genes were finally explored and visualized. Results: There was total of 382 shared DEGs between different case groups in the experiment, whereas 9 common shared DEGs were found among all three groups. The significant enrichments of 28 GO terms and 3 KEGG pathways were comprehensively analyzed with corresponding functionally enriched genes. Then, 3 key modules and 10 hub genes were further identified and explored in the resulted PPI network. In the disease-related signaling pathways, eleven potential neuropathic disease-related genes may complement the treatment of neurodegenerative diseases. Conclusion: The study found that chronic exposure to nicotine would result in the differential expression of the disease-related genes, whereas these DEGs might increase the environmental risks of Huntington's disease, Alzheimer's disease and other multiple neurodegenerative diseases.
    • Contributed Indexing:
      Keywords: Nicotine exposure; brain tissue; differentially expressed genes; neurodegenerative disease; rat brain
    • Accession Number:
      6M3C89ZY6R (Nicotine)
    • Publication Date:
      Date Created: 20241117 Date Completed: 20241118 Latest Revision: 20241117
    • Publication Date:
      20241118
    • Accession Number:
      10.3923/pjbs.2024.547.566
    • Accession Number:
      39551957