Expression of NGF, proNGF, p75 NTR in lung injury induced by cerebral ischemia-reperfusion in young and elderly rats.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 101244734 Publication Model: eCollection Cited Medium: Internet ISSN: 1980-5322 (Electronic) Linking ISSN: 18075932 NLM ISO Abbreviation: Clinics (Sao Paulo) Subsets: MEDLINE
    • Publication Information:
      Publication: 2022- : [New York] : Elsevier
      Original Publication: São Paulo, SP : Hospital das Clínicas, Faculty of Medicine, University of São Paulo, [2005]-
    • Subject Terms:
      Nerve Growth Factor*/metabolism ; Rats, Sprague-Dawley* ; Reperfusion Injury*/metabolism ; Receptors, Nerve Growth Factor*/metabolism; Animals ; Male ; Age Factors ; Lung/metabolism ; Lung/pathology ; Protein Precursors/metabolism ; Blotting, Western ; Brain Ischemia/metabolism ; Lung Injury/metabolism ; Rats ; Nerve Tissue Proteins/metabolism ; Disease Models, Animal ; Nerve Growth Factors ; Receptors, Growth Factor
    • Abstract:
      Objective: This study aims to investigate the expression levels of Nerve Growth Factor (NGF), the precursor form of NGF (proNGF), and p75 neurotrophin receptor (p75 NTR ) in lung injury induced by cerebral Ischemia-Reperfusion (I/R) in both young and elderly rats.
      Methods: Male Sprague-Dawley rats, categorized as young (3-months-old) and elderly (16-months-old), were divided into four experimental groups: Young Sham, Young I/R, Elderly Sham, and Elderly I/R. Each group underwent either sham surgery or ischemia-reperfusion treatment. Following 24 h post-procedure, the severity of cerebral ischemia was assessed using the Zea Longa 5-point scoring system, and lung tissue pathological changes were examined using Hematoxylin and Eosin (HE) staining. Western blot analysis was utilized to measure the expression levels of NGF, proNGF, and p75 NTR proteins in lung tissue.
      Results: Both young and elderly I/R groups exhibited lung tissue congestion and edema compared to their respective sham groups, with a significant increase in pathological scores (p < 0.05). Furthermore, the elderly I/R group demonstrated a significantly higher pathological score compared to the young I/R group (p < 0.05). Western blot analysis revealed that compared to the young sham group, the expression of NGF in the lung tissue of elderly sham rats decreased (p < 0.05), while proNGF and p75 NTR increased (p < 0.05). Additionally, compared to the sham group, the levels of NGF, proNGF, and p75 NTR in lung tissue were elevated in both young and elderly I/R groups of rats (p < 0.05). Moreover, the expression of proNGF and p75 NTR in lung tissue was higher in the elderly I/R group than in the young I/R group (p < 0.05).
      Conclusion: Cerebral ischemia-reperfusion-induced lung injury was associated with increased expression of proNGF and p75 NTR , as well as decreased NGF expression in lung tissue. These alterations in NGF, proNGF, and p75 NTR may contribute to the susceptibility to age-related lung injury.
      Competing Interests: Declaration of competing interest All of the authors had no any personal, financial, commercial, or academic conflicts of interest separately.
      (Copyright © 2024 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved.)
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    • Contributed Indexing:
      Keywords: Acute lung injury; Cerebral ischemia; NGF; p75(NTR); proNGF
    • Accession Number:
      9061-61-4 (Nerve Growth Factor)
      0 (Receptors, Nerve Growth Factor)
      136958-07-1 (Ngfr protein, rat)
      0 (Protein Precursors)
      0 (Nerve Tissue Proteins)
      0 (pro-nerve growth factor, rat)
      0 (Nerve Growth Factors)
      0 (Receptors, Growth Factor)
    • Publication Date:
      Date Created: 20241116 Date Completed: 20241118 Latest Revision: 20241206
    • Publication Date:
      20241209
    • Accession Number:
      PMC11609677
    • Accession Number:
      10.1016/j.clinsp.2024.100532
    • Accession Number:
      39549390