Influence of temperature changes on oxidative stress and antioxidant defense system in the bay scallop, Argopecten irradians.

Publisher: Elsevier Science Country of Publication: United States NLM ID: 9806096 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-4332 (Electronic) Linking ISSN: 10956433 NLM ISO Abbreviation: Comp Biochem Physiol A Mol Integr Physiol Subsets: MEDLINE

Original Publication: New York, : Elsevier Science, c1998-

Pectinidae*/metabolism ; Pectinidae*/physiology ; Pectinidae*/genetics ; Oxidative Stress* ; Antioxidants*/metabolism ; Hydrogen Peroxide*/metabolism ; Malondialdehyde*/metabolism ; Temperature* ; Superoxide Dismutase*/metabolism ; Catalase*/metabolism ; HSP70 Heat-Shock Proteins*/metabolism ; HSP70 Heat-Shock Proteins*/genetics; Animals ; Glutathione Transferase/metabolism ; Glutathione Transferase/genetics

In this study, we aimed to understand the effects of changes in temperature on biochemical and molecular responses associated with the antioxidant defense system in the bay scallop, Argopecten irradians. We measured the contents of H 2 O 2 and malondialdehyde (MDA), as well as the activities of antioxidant enzymes (e.g., glutathione S-transferase [GST], superoxide dismutase [SOD], and catalase [CAT]), and the regulation of stress-related genes (e.g., GST, SOD, CAT, and heat shock protein 70 [HSP70]). In addition, total antioxidant capacity (TAC) was examined in scallops exposed to different temperatures. A. irradians showed high levels of H 2 O 2 and MDA in response to acute thermal stress (48 and 72 h of exposure). Temperature changes also led to a significant increase in antioxidant enzyme activity and mRNA expression levels in A. irradians. Interestingly, the TAC increased in response to acute thermal stress (28 °C) for up to 12 h and decreased thereafter. The oxidative stress induced by high temperatures could not be alleviated by an increase in levels of antioxidant enzymes, such as GST, SOD, and CAT, resulting in high levels of H 2 O 2 and MDA and low levels of TAC. In addition, significant changes (P < 0.05) in HSP70 levels were observed in response to changes in temperature, suggesting that HSP70 played an important role in the heat tolerance of A. irradians. In conclusion, A. irradians exhibits a greater degree of oxidative stress responses in high-temperature environments than that in low-temperature environments. Overall, these findings indicate that temperature changes lead to oxidative stress, resulting in cellular damage and activation of the antioxidant defense system in bay scallops. Further experiments are required to elucidate other antioxidants and fully understand the redox system in A. irradians.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)

Keywords: Catalase; Glutathione S-transferase; Reactive oxygen species; Superoxide dismutase; Thermal stress

0 (Antioxidants)
BBX060AN9V (Hydrogen Peroxide)
4Y8F71G49Q (Malondialdehyde)
EC 1.15.1.1 (Superoxide Dismutase)
EC 1.11.1.6 (Catalase)
0 (HSP70 Heat-Shock Proteins)
EC 2.5.1.18 (Glutathione Transferase)

Date Created: 20241113 Date Completed: 20241123 Latest Revision: 20241123

20241126

10.1016/j.cbpa.2024.111775

39537095