Transforming growth factor β-2 is rhythmically expressed in both WT and BMAL1-deficient hypothalamic neurons and regulates neuropeptide Y: Disruption by palmitate.

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  • Additional Information
    • Source:
      Publisher: North Holland Publishing Country of Publication: Ireland NLM ID: 7500844 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8057 (Electronic) Linking ISSN: 03037207 NLM ISO Abbreviation: Mol Cell Endocrinol Subsets: MEDLINE
    • Publication Information:
      Publication: Limerick : North Holland Publishing
      Original Publication: Amsterdam, North-Holland.
    • Subject Terms:
      Hypothalamus*/metabolism ; ARNTL Transcription Factors*/genetics ; ARNTL Transcription Factors*/metabolism ; ARNTL Transcription Factors*/deficiency ; Neurons*/metabolism ; Neuropeptide Y*/metabolism ; Neuropeptide Y*/genetics ; Transforming Growth Factor beta2*/metabolism ; Transforming Growth Factor beta2*/genetics ; Circadian Rhythm*/genetics; Animals ; Mice ; Mice, Knockout ; Gene Expression Regulation/drug effects ; Palmitates/pharmacology ; Mice, Inbred C57BL ; Cells, Cultured
    • Abstract:
      The hypothalamus contains neuropeptide Y (NPY)-expressing neurons that control food intake and regulate energy homeostasis. During the development of obesity, neuroinflammation occurs in the hypothalamus before peripheral tissues, but the cytokines involved have not been thoroughly studied. Among them is the transforming growth factor beta (TGF-β) family of cytokines. Herein, we demonstrate that Tgfb 1-3, as well as its receptors Tgfbr1 and Tgfbr2, exhibit high levels of expression in the whole hypothalamus, primary hypothalamic culture, and immortalized hypothalamic neurons. Of interest, only Tgfb2 mRNA displays circadian expression in the immortalized hypothalamic neurons and maintains this rhythmicity in BMAL1-KO-derived hypothalamic neurons that are deficient of inherent clock gene rhythmicity. Although BMAL2 may serve as an alternative rhythm generation mechanism in the absence of BMAL1, its knockdown did not affect Tgfb2 expression. Treatment of immortalized NPY-expressing neurons with TGF-β2 upregulates the core circadian oscillators Bmal1 and Nr1d1, and importantly, also Npy mRNA expression. With obesity, the hypothalamus is exposed to elevated levels of palmitate, a saturated fatty acid that promotes neuroinflammation by upregulating pro-inflammatory cytokines. Palmitate treatment disrupts the expression of TGF-β signaling components, increases BMAL1 binding to the Tgfb2 5' regulatory region, and upregulates Npy mRNA, whereas antagonizing TGFBRI attenuates the upregulation of Npy. These results suggest that hypothalamic neuronal TGF-β2 lies at the intersection of circadian rhythms, feeding neuropeptide control, and neuroinflammation. A better understanding of the underlying mechanisms that link nutrient excess to hypothalamic dysfunction is critical for the development of effective prevention and treatment strategies.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Circadian rhythm; Hypothalamus; Neuroinflammation; Neuropeptide Y; Palmitate; Transforming growth factor beta
    • Accession Number:
      0 (ARNTL Transcription Factors)
      0 (Neuropeptide Y)
      0 (Transforming Growth Factor beta2)
      0 (Bmal1 protein, mouse)
      0 (Palmitates)
    • Publication Date:
      Date Created: 20241110 Date Completed: 20241128 Latest Revision: 20241128
    • Publication Date:
      20241202
    • Accession Number:
      10.1016/j.mce.2024.112411
    • Accession Number:
      39522861