CRISPR-Cas12a-regulated DNA adsorption on MoS 2 quantum dots: Enhanced enzyme mimics for sensitive colorimetric detection of human monkeypox virus and human papillomavirus DNA.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Oxford : Pergamon Press
    • Subject Terms:
      Quantum Dots*/chemistry ; Colorimetry*/methods ; Molybdenum*/chemistry ; Disulfides*/chemistry ; DNA, Viral*/genetics; Humans ; Adsorption ; Biosensing Techniques/methods ; CRISPR-Cas Systems ; Papillomaviridae/genetics ; Limit of Detection ; Alphapapillomavirus/genetics ; Endodeoxyribonucleases/chemistry ; Endodeoxyribonucleases/metabolism ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Human Papillomavirus Viruses ; CRISPR-Associated Proteins
    • Abstract:
      Diseases caused by viruses, such as monkeypox virus (MPXV) and human papillomavirus (HPV), pose serious threats to human health and safety. Although numerous strategies have been constructed for detecting MPXV and HPV DNA, most methods require either laborious procedures or complicated instruments involving skilled professionals. In this research, a CRISPR-Cas12a-mediated colorimetric detection platform for MPXV and HPV sensing was constructed for the first time by applying probe DNA to reprogram the catalytic properties of molybdenum disulfide quantum dots (MoS 2 QDs). In the presence of MPXV or HPV targets, the CRISPR-Cas12a trans-cleavage activity is effectively motivated to decompose the probe DNA, leading to the suppression of enzymatic activity DNA enhancer adsorbed on MoS 2 QDs, resulting in greatly decreased catalytic behaviors. The MoS 2 QDs-DNA nanohybrids displayed prominent specificity and sensitivity, with detection limits at subpicomolar levels, as well as excellent stability and accuracy for determining MPXV and HPV DNA in human sera biosamples. Furthermore, the proposed colorimetric biosensing approach can be ensembled with a smartphone platform, allowing visible analysis of DNA targets. Taken together, this colorimetric strategy offers a novel diagnosis method for MPXV and HPV DNA detection, particularly favorable for highly endemic developing countries with restricted medical and instrumental support.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: CRISPR-Cas12a; Colorimetric detection; Enzyme mimics; Human papillomavirus; Molybdenum disulfide quantum dots; Monkeypox virus
    • Accession Number:
      81AH48963U (Molybdenum)
      ZC8B4P503V (molybdenum disulfide)
      0 (Disulfides)
      0 (DNA, Viral)
      EC 3.1.- (Cas12a protein)
      EC 3.1.- (Endodeoxyribonucleases)
      0 (Bacterial Proteins)
      0 (CRISPR-Associated Proteins)
    • Publication Date:
      Date Created: 20241109 Date Completed: 20241210 Latest Revision: 20241210
    • Publication Date:
      20250114
    • Accession Number:
      10.1016/j.talanta.2024.127153
    • Accession Number:
      39520917