Identifying Calmodulin and Calmodulin-like Protein Members in Canavalia rosea and Exploring Their Potential Roles in Abiotic Stress Tolerance.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Calmodulin*/metabolism ; Calmodulin*/genetics ; Stress, Physiological*/genetics ; Phylogeny* ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Gene Expression Regulation, Plant*

Calmodulins (CaMs) and calmodulin-like proteins (CMLs) belong to families of calcium-sensors that act as calcium ion (Ca ²⁺ ) signal-decoding proteins and regulate downstream target proteins. As a tropical halophyte, Canavalia rosea shows great resistance to multiple abiotic stresses, including high salinity/alkalinity, extreme drought, heat, and intense sunlight. However, investigations of calcium ion signal transduction involved in the stress responses of C. rosea are limited. The CaM and CML gene families have been identified and characterized in many other plant species. Nevertheless, there is limited available information about these genes in C. rosea . In this study, a bioinformatic analysis, including the gene structures, conserved protein domains, phylogenetic relationships, chromosome distribution, and gene synteny, was comprehensively performed to identify and characterize CrCaMs and CrCMLs . A spatio-temporal expression assay in different organs and environmental conditions was then conducted using the RNA sequencing technique. Additionally, several CrCaM and CrCML members were then cloned and functionally characterized using the yeast heterogeneous expression system, and some of them were found to change the tolerance of yeast to heat, salt, alkalinity, and high osmotic stresses. The results of this study provide a foundation for understanding the possible roles of the CrCaM and CrCML genes, especially for halophyte C. rosea 's natural ecological adaptability for its native habitats. This study also provides a theoretical basis for further study of the physiological and biochemical functions of plant CaM s and CML s that are involved in tolerance to multiple abiotic stresses.

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32270380 the National Natural Sciences Foundation of China; 2024B1212050007 the Guangdong Science and Technology Program; GML2019ZD0408 the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou); ISEE2021ZD04 the funding from the Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences

Keywords: Canavalia rosea; calmodulin; calmodulin-like protein; ecological adaptability; yeast heterogeneous expression

0 (Calmodulin)
0 (Plant Proteins)

Date Created: 20241109 Date Completed: 20241109 Latest Revision: 20241116

20241116

PMC11545983

10.3390/ijms252111725

39519274