Thermal Bed Design for Temperature-Controlled DNA Amplification Using Optoelectronic Sensors.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101204366 Publication Model: Electronic Cited Medium: Internet ISSN: 1424-8220 (Electronic) Linking ISSN: 14248220 NLM ISO Abbreviation: Sensors (Basel) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, c2000-
    • Subject Terms:
    • Abstract:
      Loop-Mediated Isothermal Loop-Mediated Isothermal Amplification (LAMP) is a widely used technique for nucleic acid amplification due to its high specificity, sensitivity, and rapid results. Advances in microfluidic lab-on-chip (LOC) technology have enabled the integration of LAMP into miniaturized devices, known as μ-LAMP, which require precise thermal control for optimal DNA amplification. This paper introduces a novel thermal bed design using PCB copper traces and FR-4 dielectric materials, providing a reliable, modular, and repairable heating platform. The system achieves accurate and stable temperature control, which is critical for μ-LAMP applications, with temperature deviations within ±1.0 °C. The thermal bed's performance is validated through finite element method (FEM) simulations, showing uniform temperature distribution and a rapid thermal response of 2.5 s to reach the target temperature. These results highlight the system's potential for applications such as disease diagnostics, biological safety, and forensic analysis, where precision and reliability are paramount.
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    • Contributed Indexing:
      Keywords: DNA amplification; LAMP; microfluidics; optoelectronic sensors; thermal control
    • Accession Number:
      9007-49-2 (DNA)
    • Subject Terms:
      LAMP assay
    • Publication Date:
      Date Created: 20241109 Date Completed: 20241109 Latest Revision: 20241116
    • Publication Date:
      20241116
    • Accession Number:
      PMC11548344
    • Accession Number:
      10.3390/s24217050
    • Accession Number:
      39517946