A novel sensing probe based on AuNPs-Apt for the detection of enrofloxacin.

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  • Additional Information
    • Source:
      Publisher: IOP Pub Country of Publication: England NLM ID: 101241272 Publication Model: Electronic Cited Medium: Internet ISSN: 1361-6528 (Electronic) Linking ISSN: 09574484 NLM ISO Abbreviation: Nanotechnology Subsets: MEDLINE
    • Publication Information:
      Original Publication: Bristol, UK : IOP Pub., c1990-
    • Subject Terms:
      Enrofloxacin*/analysis ; Gold*/chemistry ; Metal Nanoparticles*/chemistry ; Aptamers, Nucleotide*/chemistry ; Biosensing Techniques*/methods ; Fluoroquinolones*/analysis ; Fluoroquinolones*/pharmacology ; Anti-Bacterial Agents*/analysis ; Anti-Bacterial Agents*/pharmacology; Limit of Detection
    • Abstract:
      Enrofloxacin (ENR), as a synthetic broad-spectrum antibiotic is widely utilized in veterinary medicine to treat animal diseases and promote livestock growth, it can inhibit bacterial DNA gyrase subunit A, thereby preventing bacterial DNA replication and exerting its antibacterial effect. However, excessive use of enrofloxacin poses significant risks to ecological balance and human health due to residual contamination. We have developed a novel ENR aptamer sensor based on the gold nanoparticles/aptamer (AuNPs-Apt) complexes, in which AuNPs were synthesized via the seed method and functionalized with aptamers. The optical properties, particle size, functional groups and morphology of the AuNPs-Apt probe were characterized by transmission electron microscope, Fourier transform infrared spectrometer and UV-vis spectrophotometer, respectively. The aptamer biosensor can specifically identify enrofloxacin, with a wide detection range (0.05-100 μ g ml -1 ) and a good linear relationship (R2=0.99) within the detection range. In addition, the biosensor also has the advantages of short detection time, low biological toxicity, good stability, and low detection cost. Therefore, it shows a great prospect for practical application in the field of detecting enrofloxacin residues.
      (© 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)
    • Contributed Indexing:
      Keywords: antibiotic residue monitoring; enrofloxacin; gold nanoparticles/aptamer complexes; sensing probe
    • Accession Number:
      3DX3XEK1BN (Enrofloxacin)
      7440-57-5 (Gold)
      0 (Aptamers, Nucleotide)
      0 (Fluoroquinolones)
      0 (Anti-Bacterial Agents)
    • Publication Date:
      Date Created: 20241108 Date Completed: 20241108 Latest Revision: 20241108
    • Publication Date:
      20241114
    • Accession Number:
      10.1088/1361-6528/ad8c4b
    • Accession Number:
      39511915