Protective immune-response of Aeromonas hydrophila phage lysate in crucian carp against direct virulent challenge with A. hydrophila-TPS.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London ; New York : Academic Press, c1991-
    • Subject Terms:
      Aeromonas hydrophila*/physiology ; Aeromonas hydrophila*/immunology ; Carps*/immunology ; Bacterial Vaccines*/immunology ; Bacteriophages*/immunology ; Bacteriophages*/physiology ; Fish Diseases*/immunology ; Fish Diseases*/prevention & control ; Gram-Negative Bacterial Infections*/veterinary ; Gram-Negative Bacterial Infections*/immunology ; Gram-Negative Bacterial Infections*/prevention & control; Animals
    • Abstract:
      Aeromonas hydrophila was a common opportunistic pathogen that was widely found in various aquatic environment and could cause multiple infections in humans and animals. The haemorrhagic septicemia and bacterial enteritis in fish triggered by this pathogen led to significant losses in the aquaculture industry. In this study, we aimed to develop a phage lysate vaccine by lysing the A. hydrophila-TPS strain using phage PZY-Ah, which was previously isolated and preserved in the laboratory. First, we focused on optimizing phage lysis conditions, including different host bacteria culture time, phage lysis time, and phage to bacterial ratios. The optimal conditions were established as follows: culturing the A. hydrophila-TPS strain for 6 h, adding phage at a ratio of 1:10, and mixing for 4 h, which resulted in maximum lysis of the host bacteria. Subsequently, we assessed the immune responses of groups receiving formaldehyde-inactivated vaccines compared to those receiving various concentrations of the phage lysate vaccine. Both the TPS-phage lysate and formaldehyde-inactivated vaccine groups exhibited increased levels of specific immune enzymes (ACP, AKP, LZM, SOD, CAT) and cytokines (IL-1β, TNF-α and IFN-γ) in serum, as well as enhanced humoral immunity (IgM, C3 and C4) in crucian carp. Furthermore, challenge tests conducted post-immunization demonstrated that the high concentration of the TPS strain-lysate vaccine group (1 × 10 8  CFU/mL) achieved the highest immune protection rate at 88.89 %. Overall, the development of the TPS-phage lysate vaccine significantly enhanced the immunity of crucian carp, providing a higher level of protection and establishing a foundation for the research and development of phage-based aquatic vaccines.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Aeromonas hydrophila; Crucian carp; Immune protection; Phage lysate vaccine
    • Accession Number:
      0 (Bacterial Vaccines)
    • Publication Date:
      Date Created: 20241107 Date Completed: 20241201 Latest Revision: 20241201
    • Publication Date:
      20241204
    • Accession Number:
      10.1016/j.fsi.2024.110011
    • Accession Number:
      39510428