Engineering Halomonas bluephagenesis for synthesis of polyhydroxybutyrate (PHB) in the presence of high nitrogen containing media.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: Belgium NLM ID: 9815657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-7184 (Electronic) Linking ISSN: 10967176 NLM ISO Abbreviation: Metab Eng Subsets: MEDLINE
    • Publication Information:
      Original Publication: Brugge, Belgium ; Orlando, FL : Academic Press, c1999-
    • Subject Terms:
      Halomonas*/metabolism ; Halomonas*/genetics ; Halomonas*/growth & development ; Hydroxybutyrates*/metabolism ; Nitrogen*/metabolism ; Metabolic Engineering*; Polyesters/metabolism ; Bacterial Proteins/metabolism ; Bacterial Proteins/genetics
    • Abstract:
      The trade-offs exist between microbial growth and bioproduct synthesis including intracellular polyester polyhydroxybutyrate (PHB). Under nitrogen limitation, more carbon flux is directed to PHB synthesis while growth is inhibited with diminishing overall carbon utilization, similar to the suboptimal carbon utilization during glycolysis-derived pyruvate decarboxylation. This study reconfigured the central carbon network of Halomonas bluephagenesis to improve PHB yield theoretically and practically. It was found that the downregulation of glutamine synthetase (GS) activity led to a synchronous improvement on PHB accumulation and cell growth under nitrogen non-limitation condition, increasing the PHB yield from glucose (g/g) to 85% of theoretical yield, PHB titer from 7.6 g/L to 12.9 g/L, and from 51 g/L to 65 g/L when grown in shake flasks containing a rich N-source, and grown in a fed-batch cultivation conducted in a 7-L bioreactor also containing a rich N-source, respectively. Results offer better metabolic balance between glucose conversion efficiency and microbial growth for economic PHB production.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. .
      (Copyright © 2024 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Glutamine synthetase; Halomonas; Metabolic engineering; Next generation industrial biotechnology; Nitrogen metabolism; PHB; Synthetic biology
    • Accession Number:
      0 (Hydroxybutyrates)
      N762921K75 (Nitrogen)
      0 (Polyesters)
      0 (Bacterial Proteins)
    • Publication Date:
      Date Created: 20241103 Date Completed: 20241202 Latest Revision: 20241202
    • Publication Date:
      20241204
    • Accession Number:
      10.1016/j.ymben.2024.10.012
    • Accession Number:
      39490668