EB-SUN, a new microtubule plus-end tracking protein in Drosophila .

Publisher: American Society for Cell Biology Country of Publication: United States NLM ID: 9201390 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-4586 (Electronic) Linking ISSN: 10591524 NLM ISO Abbreviation: Mol Biol Cell Subsets: MEDLINE

Original Publication: Bethesda, MD : American Society for Cell Biology, c1992-

Microtubules*/metabolism ; Microtubule-Associated Proteins*/metabolism ; Drosophila Proteins*/metabolism ; Drosophila Proteins*/genetics ; Oogenesis*/physiology ; Oocytes*/metabolism ; Drosophila melanogaster*/metabolism ; Drosophila melanogaster*/embryology; Animals ; Female ; Ovary/metabolism ; Germ Cells/metabolism ; Drosophila/metabolism

Microtubule (MT) regulation is essential for oocyte development. In Drosophila , MT stability, polarity, abundance, and orientation undergo dynamic changes across developmental stages. In our effort to identify novel microtubule-associated proteins that regulate MTs in the Drosophila ovary, we identified a previously uncharacterized gene, CG18190, which encodes a novel MT end-binding (EB) protein, which we propose to name EB-SUN. We show that EB-SUN colocalizes with EB1 at growing MT plus-ends in Drosophila S2 cells. Tissue-specific and developmental expression profiles from Paralog Explorer reveal that EB-SUN is predominantly expressed in the ovary and early embryos, while EB1 is ubiquitously expressed. Furthermore, as early as oocyte determination, EB-SUN comets are highly concentrated in oocytes during oogenesis. EB-SUN knockout (KO) results in decreased MT density at the onset of mid-oogenesis (stage 7) and delays oocyte growth during late mid-oogenesis (stage 9). Combining EB-SUN KO with EB1 knockdown (KD) in germ cells significantly further reduces MT density at stage 7. Hatching assays of single protein depletion reveal distinct roles for EB-SUN and EB1 in early embryogenesis, likely due to differences in their expression and binding partners. Notably, all eggs from EB-SUN KO/EB1 KD females fail to hatch, suggesting partial redundancy between these proteins.
Competing Interests: Conflicts of interest: The authors declare no financial conflict of interest.

Update of: bioRxiv. 2024 Sep 11:2024.09.11.612465. doi: 10.1101/2024.09.11.612465. (PMID: 39314338)

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P40 OD018537 United States OD NIH HHS; R35 GM131752 United States GM NIGMS NIH HHS

0 (Microtubule-Associated Proteins)
0 (Drosophila Proteins)
0 (EB1 microtubule binding proteins)

Date Created: 20241030 Date Completed: 20241108 Latest Revision: 20250104

20250104

PMC11656466

10.1091/mbc.E24-09-0402

39475714