Validation of a triiodothyronine (T3) ELISA for mouse fecal samples.

Publisher: published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society Country of Publication: United States NLM ID: 101607800 Publication Model: Print Cited Medium: Internet ISSN: 2051-817X (Electronic) Linking ISSN: 2051817X NLM ISO Abbreviation: Physiol Rep Subsets: MEDLINE

Original Publication: [Malden MA] : published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society, 2013-

Feces*/chemistry ; Triiodothyronine*/blood ; Enzyme-Linked Immunosorbent Assay*/methods; Animals ; Mice ; Male ; Mice, Inbred C57BL ; Methimazole/pharmacology ; Female

Acquiring sufficient blood for hormone analysis in mice can be a limiting step. Hormone analysis techniques using non-invasive sample collection have been vigorously developed for endangered species, from whom blood sampling is prohibited, or from species that are otherwise difficult to handle in a laboratory setting. Because there are interactions between glucocorticoids and thyroid hormones (T3 and T4), reducing the animal's "distress" during sample collection is imperative. Measurement of fecal T3 provides less sensitive, baseline information regarding thyroid function while permitting a non-invasive technique for more frequent sampling. We demonstrated that using a methanol extraction protocol produced the most reliable fecal T3 measurement in an enzyme-linked immunosorbent assay (ELISA). We found that during a thyroid hormone-treated state, fecal and plasma T3 measurements from mice are directly related, while during a methimazole-treated state, fecal and plasma T3 measurements from mice are inversely related. Fecal samples are a useful way to monitor thyroid hormone function in laboratory mice.
(© 2024 The Author(s). Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)

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Southern Illinois University Edwardsville Graduate School STEP; AIA23-02 Southern Illinois University School of Dental Medicine; Southern Illinois University Edwardsville URCA

Keywords: fecal extracts; mice; non‐invasive sampling; periodontal disease; thyroid hormones

06LU7C9H1V (Triiodothyronine)
554Z48XN5E (Methimazole)

Date Created: 20241029 Date Completed: 20241029 Latest Revision: 20241101

20241101

PMC11521789

10.14814/phy2.70115

39472298