Vasculo-osteogenic keratin-based nanofibers containing merwinite nanoparticles and sildenafil for bone tissue regeneration.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
    • Subject Terms:
      Nanofibers*/chemistry ; Bone Regeneration*/drug effects ; Osteogenesis*/drug effects ; Sildenafil Citrate*/pharmacology ; Sildenafil Citrate*/administration & dosage ; Sildenafil Citrate*/chemistry ; Keratins*/chemistry ; Tissue Scaffolds*/chemistry ; Nanoparticles*/chemistry ; Tissue Engineering*/methods; Animals ; Neovascularization, Physiologic/drug effects ; Chorioallantoic Membrane/drug effects ; Drug Liberation ; Polyethylene Glycols/chemistry ; Tensile Strength ; Bone and Bones/drug effects
    • Abstract:
      Vascularization of bone tissue constructs plays a pivotal role in facilitating nutrient transport and metabolic waste removal during the processes of osteogenesis and bone regeneration in vivo. In this study, a sildenafil (Sil)-loaded nanofibrous scaffold of keratin/Soluplus/merwinite (KS.Me.Sil) was fabricated through electrospinning and the effectiveness of the scaffold was assessed for bone tissue engineering applications. The KS.Me.Sil nanofibrous scaffold exhibited notably enhanced ultimate tensile strength (3.38 vs 2.61 MPa) and elastic modulus (69.83 vs 46.27 MPa) compared to the KS scaffold. The in vitro release of Ca 2+ , Si 4+ and Mg 2+ ions and the release of Sil from the nanofibers as well as biodegradability and bioactivity were evaluated for 14 days. Protein adsorption capability and cytocompatibility of the scaffolds were tested. Alkaline phosphatase activity test, Alizarin red staining and qRT-PCR analysis demonstrated that the KS.Me.Sil nanofibers had the best osteogenic activity among other samples. Also, the results of the chorioallantoic membrane assay showed an almost threefold increase in blood vessel density in the group treated with the KS.Me.Sil nanofibers extract compared to the KS. In conclusion, our findings suggest that the electrospun KS.Me.Sil nanofibrous scaffold offers a robust structure with exceptional osteogenic and angiogenic characteristics, making it a promising candidate for bone tissue engineering applications.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Electrospinning; In ovo vascularization; Keratin; Merwinite; Osteogenic differentiation; Sildenafil
    • Accession Number:
      BW9B0ZE037 (Sildenafil Citrate)
      68238-35-7 (Keratins)
      3WJQ0SDW1A (Polyethylene Glycols)
    • Publication Date:
      Date Created: 20241029 Date Completed: 20241123 Latest Revision: 20241123
    • Publication Date:
      20241126
    • Accession Number:
      10.1016/j.ijpharm.2024.124875
    • Accession Number:
      39471889