APOBR Is Downregulated in EBV+ Tonsils of Children with Obstructive Sleep-Disordered Breathing.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel : MDPI
    • Subject Terms:
      Palatine Tonsil*/virology ; Palatine Tonsil*/metabolism ; Herpesvirus 4, Human*/genetics ; Sleep Apnea, Obstructive*/genetics ; Sleep Apnea, Obstructive*/virology ; Epstein-Barr Virus Infections*/virology ; Epstein-Barr Virus Infections*/genetics ; Epstein-Barr Virus Infections*/complications; Humans ; Child ; Female ; Male ; Child, Preschool ; Down-Regulation ; Continuous Positive Airway Pressure ; Adolescent ; Adult ; Biomarkers
    • Abstract:
      Background: Obstructive sleep-disordered breathing (oSDB) is a heterogeneous phenotype that is increasing in prevalence worldwide and has many potential comorbidities that could severely affect quality of life. There is a need to identify biomarkers for oSDB and its comorbidities to improve clinical management, particularly in children. Methods: We performed bulk mRNA-sequencing, differential expression analysis, and qPCR replication of selected differentially expressed genes (DEGs) using RNA samples extracted from tonsils of children with oSDB. Two variables were used as classifier, namely, detection of Epstein-Barr virus (EBV) in tonsils and need for continuous positive airway pressure (CPAP) treatment. Standard statistical tests were used to determine associations across clinical, EBV, and DEG variables. Results: Nineteen genes were dysregulated in tonsils that are EBV+ or from children needing CPAP. Of these genes, APOBR was downregulated in both EBV+ and CPAP+ tonsils, and this downregulation was replicated by qPCR in an independent set of pediatric samples. In the tonsils of adult patients with oSDB, APOBR was positively correlated with age, and potentially with diastolic blood pressure. Conclusion s: Taken together, APOBR and DEGs in tonsillar tissues may be useful as potential biomarkers of oSDB severity and comorbidity across the lifespan, with APOBR levels being dependent on latent EBV infection.
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    • Grant Information:
      T32 DC012280 United States DC NIDCD NIH HHS; R25 HG012994 United States HG NHGRI NIH HHS
    • Contributed Indexing:
      Keywords: APOBR; CPAP; EBV; Epstein–Barr virus; RNA-seq; bulk mRNA sequencing; continuous positive airway pressure; obstructive sleep-disordered breathing; sleep apnea; tonsil
    • Accession Number:
      0 (Biomarkers)
    • Publication Date:
      Date Created: 20241026 Date Completed: 20241026 Latest Revision: 20241104
    • Publication Date:
      20241104
    • Accession Number:
      PMC11507010
    • Accession Number:
      10.3390/genes15101324
    • Accession Number:
      39457448