Stability characterizations of feed-based bivalent vaccine containing inactivated Streptococcus agalactiae and Aeromonas hydrophila against streptococcosis and Aeromonas infections in red hybrid tilapia (Oreochromis sp.).

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-072X (Electronic) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE

Original Publication: Berlin, New York, Springer-Verlag.

Aeromonas hydrophila*/immunology ; Streptococcus agalactiae*/immunology ; Fish Diseases*/prevention & control ; Fish Diseases*/microbiology ; Fish Diseases*/immunology ; Gram-Negative Bacterial Infections*/prevention & control ; Gram-Negative Bacterial Infections*/veterinary ; Gram-Negative Bacterial Infections*/immunology ; Streptococcal Infections*/prevention & control ; Streptococcal Infections*/veterinary ; Streptococcal Infections*/microbiology ; Streptococcal Infections*/immunology ; Bacterial Vaccines*/immunology ; Bacterial Vaccines*/administration & dosage ; Bacterial Vaccines*/chemistry; Animals ; Tilapia/microbiology ; Tilapia/immunology ; Animal Feed/analysis ; Animal Feed/microbiology ; Vaccines, Inactivated/immunology

Feed-based bivalent vaccine (FBBV) containing killed whole organism (KWO) of Streptococcus agalactiae and Aeromonas hydrophila with 10% palm oil was previously proved to improve red hybrid tilapia's (Oreochromis sp.) immunity against streptococcosis and Aeromonas infections. This study characterized the FBBV's stability following the preparatory process and storage. The FBBV was prepared, and the KWO's stability was determined microscopically and molecularly. The efficacy of FBBV stored at room temperature (25 ± 2 °C) for 0, 30 and 60 days was investigated in red hybrid tilapia. The results indicated the addition of palm oil was not affecting the KWO's structure and helping in the FBBV's pelletization. In 1 g of FBBV contained 1.5 × 10 ⁹ CFU/g of S. agalactiae and 4.9 × 10 ⁹ CFU/g of A. hydrophila, respectively, even after 60 days of storage at room temperature. The KWO's structure in FBBV was not affected following in vitro acidic tolerance analysis, as noted from light and electron microscopies. The FBBV's carbohydrate, energy, moisture, total protein and total ash contents remained stable at 95% after 60 days of storage at room temperature, while the KWO's concentration was slightly reduced to 83.3% for S. agalactiae (1.25 × 10 ⁹ CFU/g) and 80.6% for A. hydrophila (3.85 × 10 ⁹ CFU/g), respectively. Fish vaccinated with FBBV that was stored for 0, 30 and 60 days did not show any significant differences (p ≥ 0.05) in the relative percent survival when challenged with pathogenic Streptococcus spp. and Aeromonas spp. These findings suggested that the FBBV is a stable vaccine, which underscores its potential application as aquatic vaccines in aquaculture.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

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GPB/2020/9694800 Geran Putra Berimpak, Universiti Putra Malaysia; 6369100 HICOE, MoHE

Keywords: Bacterial diseases; Feed-based vaccine characterizations; Stability analyses; Tilapia

0 (Bacterial Vaccines)
0 (Vaccines, Inactivated)

Date Created: 20241023 Date Completed: 20241023 Latest Revision: 20241109

20241109

10.1007/s00203-024-04166-2

39443367