Light-induced cryptochrome 2 liquid-liquid phase separation and mRNA methylation.

Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE

Publication: Oxford : Wiley on behalf of New Phytologist Trust
Original Publication: London, New York [etc.] Academic Press.

Cryptochromes*/metabolism ; Cryptochromes*/genetics ; Light* ; RNA, Messenger*/metabolism ; RNA, Messenger*/genetics; Methylation/radiation effects ; Phase Separation

Light is essential not only for photosynthesis but also for the regulation of various physiological and developmental processes in plants. While the mechanisms by which light regulates transcription and protein stability are well established, the effects of light on RNA methylation and their subsequent impact on plant growth and development are less understood. Upon exposure to blue light, the photoreceptor cryptochromes form nuclear speckles or nuclear bodies, termed CRY photobodies. The CRY2 photobodies undergo light-induced homo-oligomerization and liquid-liquid phase separation (LLPS), which are crucial for their physiological activity. Recent studies have proposed that blue light-induced CRY2 LLPS increases the local concentration or directly enhances the biochemical activities of RNA N6-methyladenosine (m ⁶ A) methyltransferases, thus, to regulate circadian clock and maintain Chl homeostasis through processes of RNA decay or translation. This review aimed to elucidate the functions of CRY2 and LLPS in RNA methylation, focusing on the light-controlled reversible phase transitions regulon and the outstanding questions that remain in RNA methylation.
(© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.)

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Keywords: CRY2 photobodies; biomolecular condensates; blue light signaling; liquid‐liquid phase separation; mRNA methylation

0 (Cryptochromes)
0 (RNA, Messenger)

Date Created: 20241022 Date Completed: 20241121 Latest Revision: 20241121

20241121

10.1111/nph.20201

39434460