Molecular insights and functional analysis of isocitrate dehydrogenase in two gram-negative pathogenic bacteria.

Publisher: Springer Country of Publication: Germany NLM ID: 9012472 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-0972 (Electronic) Linking ISSN: 09593993 NLM ISO Abbreviation: World J Microbiol Biotechnol Subsets: MEDLINE

Publication: 2005- : Berlin : Springer
Original Publication: Oxford, OX, UK : Published by Rapid Communications of Oxford Ltd in association with UNESCO and in collaboration with the International Union of Microbiological Societies, c1990-

Isocitrate Dehydrogenase*/metabolism ; Isocitrate Dehydrogenase*/genetics ; Isocitrate Dehydrogenase*/chemistry ; Klebsiella pneumoniae*/enzymology ; Klebsiella pneumoniae*/genetics ; Legionella pneumophila*/enzymology ; Legionella pneumophila*/genetics; Hydrogen-Ion Concentration ; NADP/metabolism ; NAD/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacterial Proteins/chemistry ; Molecular Weight ; Enzyme Stability ; Temperature ; Substrate Specificity ; Kinetics

Klebsiella pneumoniae and Legionella pneumophila are common Gram-negative bacteria that can cause lung infections. The multidrug resistance of K. pneumoniae presents a significant challenge for treatment. This study focuses on isocitrate dehydrogenase (IDH), a key enzyme in the oxidative metabolic pathway of these two bacteria. KpIDH and LpIDH were successfully overexpressed and purified, and their biochemical characteristics were thoroughly investigated. The study revealed that KpIDH and LpIDH are homodimeric enzymes with molecular weights of approximately 70 kDa. They are completely dependent on the coenzyme NADP ⁺ and are inactive towards NAD ⁺ . KpIDH exhibits the highest catalytic activity at pH 8.0 in the presence of Mn ²⁺ and at pH 7.8 in the presence of Mg ²⁺ . Its optimal catalytic performance is achieved with both ions at 55 °C. LpIDH exhibited its highest activity at pH 7.8 in the presence of Mn ²⁺ and Mg ²⁺ , respectively, and exhibits optimal catalytic performance at 45 °C. Heat inactivation studies showed that KpIDH and LpIDH retained over 80% of their activity after being exposed to 45 °C for 20 min. Furthermore, we successfully altered the coenzyme specificity of KpIDH and LpIDH from NADP ⁺ to NAD ⁺ by replacing four key amino acid residues. This study provides a comprehensive biochemical characterization of two multidrug-resistant bacterial IDHs commonly found in hospital environments. It enhances our understanding of the characteristics of pathogenic bacteria and serves as a reference for developing new therapeutic strategies.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)

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32071270 National Natural Science Foundation of China; 2022AH010012 Anhui Provincial Universities

Keywords: Klebsiella pneumoniae; Legionella pneumophila; Biochemical characterization; Coenzyme specificity determinants; Isocitrate dehydrogenase; Kinetics

EC 1.1.1.41 (Isocitrate Dehydrogenase)
53-59-8 (NADP)
0U46U6E8UK (NAD)
0 (Bacterial Proteins)

Date Created: 20241019 Date Completed: 20241019 Latest Revision: 20241108

20241108

10.1007/s11274-024-04169-7

39425873