Improved lymphangiogenesis around vascularized lymph node flaps by periodic injection of hyaluronidase in a rodent model.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Hyaluronoglucosaminidase*/administration & dosage ; Hyaluronoglucosaminidase*/pharmacology ; Lymphangiogenesis*/drug effects ; Lymph Nodes*/transplantation ; Rats, Sprague-Dawley* ; Lymphedema*; Animals ; Rats ; Disease Models, Animal ; Surgical Flaps ; Male ; Lymphatic Vessels/drug effects ; Fibrosis

Vascularized lymph node transfer (VLNT) is an advanced surgical approach for secondary lymphedema (SLE) treatment, but tissue fibrosis around the lymph node flap (VLNF) inhibiting lymphangiogenesis is the biggest challenge undermining its therapeutic efficacy. This study explored the effects of periodic hyaluronidase (HLD) injection in reducing fibrosis and promoting lymphangiogenesis in 52 Sprague-Dawley rats with a VLNF over 13 weeks. The results demonstrated that HLD administration significantly enhanced swelling reduction, lymphatic drainage efficiency, and lymphatic vessel regeneration, with up to a 26% decrease in tissue fibrosis around the VLNF. These findings suggest that combining VLNT with periodic injections of HLD could substantially improve SLE treatment outcomes in clinical settings. It offers a promising direction for future therapeutic strategies and drug development aimed at increasing the efficacy of surgical treatment for SLE patients.
(© 2024. The Author(s).)

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RS-2024-00338179 National Research Foundation of Korea; NRF-2023R1A2C1004544 National Research Foundation of Korea; 2021IL0037 Asan Institute for Life Sciences, Asan Medical Center

EC 3.2.1.35 (Hyaluronoglucosaminidase)

Date Created: 20241018 Date Completed: 20241018 Latest Revision: 20241023

20241023

PMC11489753

10.1038/s41598-024-74414-4

39424818