Macrophages can transmit coxsackievirus B4 to pancreatic cells and can impair these cells.

Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7705876 Publication Model: Print Cited Medium: Internet ISSN: 1096-9071 (Electronic) Linking ISSN: 01466615 NLM ISO Abbreviation: J Med Virol Subsets: MEDLINE

Publication: New York Ny : Wiley-Liss
Original Publication: New York, Liss.

Macrophages*/virology ; Enterovirus B, Human*/physiology ; Enterovirus B, Human*/drug effects; Humans ; Insulin-Secreting Cells/virology ; Insulin-Secreting Cells/drug effects ; Coxsackievirus Infections/virology ; Cell Line ; Coculture Techniques ; Cells, Cultured ; Cytokines/metabolism ; Macrophage Colony-Stimulating Factor/pharmacology

Macrophages are suspected to be involved in the pathogenesis of type 1 diabetes. The role of macrophages in the transmission of coxsackievirus B4 (CVB4) to pancreatic cells and in the alteration of these cells was investigated. Human monocytes isolated from peripheral blood were differentiated into macrophages with M-CSF (M-CSF macrophages) or GM-CSF (GM-CSF macrophages). M-CSF macrophages were inoculated with CVB4. M-CSF and GM-CSF macrophages were activated with lipopolysaccharide and interferon (IFN)-γ. Human pancreatic beta cells 1.1B4 were inoculated with CVB4 derived from M-CSF macrophages or were cocultured with CVB4-infected M-CSF macrophages. The antiviral activity of synthetic molecules in macrophage cultures was evaluated. Activated macrophages were cocultured with CVB4-persistently infected 1.1B4 cells, and the specific lysis of these cells was determined. Our study shows that CVB4 can infect M-CSF macrophages, leading to the release of interleukin-6 and tumor necrosis factor-α and later IFN-α. M-CSF macrophage-derived CVB4 can infect 1.1B4 cells, which were then altered; however, when these cells were cultured in medium containing agarose, cell layers were not altered. Fluoxetine and CUR-N373 can inhibit CVB4 replication in macrophage cultures. Supernatants of activated M-CSF and GM-CSF macrophage cultures induced lysis of CVB4-persistently infected 1.1B4 cells. The cytolytic activity of activated GM-CSF macrophages was higher towards CVB4-persistently infected 1.1B4 cells than mock-infected 1.1B4 cells. In conclusion, macrophages may play a role in CVB4 infection of pancreatic cells, and are capable of inducing lysis of infected pancreatic cells.
(© 2024 The Author(s). Journal of Medical Virology published by Wiley Periodicals LLC.)

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Keywords: CUR‐N373; coxsackievirus B4; cytolytic activity; fluoxetine; macrophages; pancreatic cells

0 (Cytokines)
81627-83-0 (Macrophage Colony-Stimulating Factor)

Date Created: 20241018 Date Completed: 20241018 Latest Revision: 20241018

20241018

10.1002/jmv.70009

39422382