Identification, expression, and function analysis of Rbpms2 splicing variants in Japanese flounder gonad.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 0370735 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-6840 (Electronic) Linking ISSN: 00166480 NLM ISO Abbreviation: Gen Comp Endocrinol Subsets: MEDLINE
    • Publication Information:
      Publication: New York, NY : Academic Press
      Original Publication: New York.
    • Subject Terms:
      Flounder*/genetics ; Flounder*/metabolism; Animals ; Female ; Male ; Gonads/metabolism ; Fish Proteins/genetics ; Fish Proteins/metabolism ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/metabolism ; Ovary/metabolism ; Testis/metabolism ; Phylogeny
    • Abstract:
      Rbpms2, an RNA-binding protein with multiple splicing (Rbpms), can interact with RNAs to involve oocyte development, thereby influencing female sex differentiation in vertebrates. Here, two splicing variants of the Rbpms2 gene from Japanese flounder (Paralichthys olivaceus) were identified, namely Rbpms2.1 and Rbpms2.2. The two variants exhibited 98.22 % amino acid homology, both featuring an RNA recognition motif (RRM) domain spanning positions 98-170 amino acids. They were relatively conserved throughout phylogenetic evolution. Differently, the C-terminal region of the Rbpms2.1 contains five additional sequential amino acids (-VRDQP-) compared to Rbpms2.2. The real-time qPCR results demonstrated that Rbpms2.1 and Rbpms2.2 had relatively abundant expression in the gonads of adult Japanese flounder, with higher expression levels in the ovary compared to the testis (P < 0.05). In situ hybridization results showed strong positive expression of Rbpms2 mRNA in oocytes at stages I-III during the V stage of ovarian development. In the testis atstage IV, the expression of Rbpms2 mRNA was mainly concentrated on primary spermatocytes. Importantly, Rbpms2 binding sites were found in the 3'UTR, 5'UTR, and ORF regions of the sex-related genes including dmrt1, sox9, amh, foxl2, and wnt4. siRNA interference and overexpression analysis of Rbpms2.1 and Rbpms2.2 in primary cells of the ovary and testis showed that Rbpms2 can repress the expression of male-related genes (dmrt1, sox9, and amh) and significantly promote the expression of female-related genes (foxl2 and wnt4). Our results revealed that Rbpms2 may play a critical role by targeting the sex-related genes in the gonad development of Japanese flounder.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024. Published by Elsevier Inc.)
    • Contributed Indexing:
      Keywords: Gonad development; Japanese flounder (Paralichthys olivaceus); Rbpms2 gene; Tissue expression
    • Accession Number:
      0 (Fish Proteins)
      0 (RNA-Binding Proteins)
    • Publication Date:
      Date Created: 20241016 Date Completed: 20241107 Latest Revision: 20241112
    • Publication Date:
      20241114
    • Accession Number:
      10.1016/j.ygcen.2024.114628
    • Accession Number:
      39414089