Differential Fatty Acid Response of Resident Macrophages in Human Skeletal Muscle Fiber and Intermuscular Adipose Tissue.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • Subject Terms:
      Macrophages*/metabolism ; Adipose Tissue*/metabolism ; Adipose Tissue*/cytology ; Muscle Fibers, Skeletal*/metabolism ; Fatty Acids*/metabolism; Humans ; Male ; Female ; Middle Aged ; Adult ; Cytokines/metabolism ; Mitochondria/metabolism ; Aged ; Muscle, Skeletal/metabolism ; Muscle, Skeletal/cytology
    • Abstract:
      Human skeletal muscle contains different types of tissues with skeletal muscle fibers (SMFs) and intermuscular adipose tissues (IMATs) as the main components. We maintained human skeletal muscle tissues from 12 study participants under native conditions in vitro for 11 days to investigate the dynamics of macrophages that reside in adjacent IMATs and SMFs simultaneously. The samples were subjected to immunohistochemical analysis for macrophage phenotyping and mitochondrial mass assessment before and after maintenance in vitro. Multiplex protein analysis was used to determine cytokine/chemokine expression in tissue extracts. The results revealed significant correlations between donor age or body mass index (BMI) and distinct phenotypes of resident macrophages in SMFs and IMATs. The dynamics of SMF- and IMAT-resident macrophages differed significantly in vitro and exhibited inverse correlations with chemokine/cytokine expression levels and mitochondrial activity. Moreover, the responses of macrophages to saturated and unsaturated fatty acids (FAs) differed substantially between SMFs and IMATs. These findings showed the functional diversity of phenotypically identical macrophages in adjacent niches. Thus, the currently available macrophage markers cannot capture the functional diversity of human tissue-resident macrophages. The model used in the present study may help elucidate how macrophages affect muscle homeostasis and disease in humans.
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    • Grant Information:
      P-068 Stiftung zur Förderung der Erforschung von Ersatz- und Ergänzungsmethoden zur Einschränkung von Tierversuchen; 202206170014 China Scholarship Council
    • Contributed Indexing:
      Keywords: adipocytes; chemokines; cytokines; fatty acids; human; mitochondria; skeletal muscle; tissue model; tissue-resident macrophages
    • Accession Number:
      0 (Fatty Acids)
      0 (Cytokines)
    • Publication Date:
      Date Created: 20241016 Date Completed: 20241016 Latest Revision: 20241019
    • Publication Date:
      20241019
    • Accession Number:
      PMC11477279
    • Accession Number:
      10.3390/ijms251910722
    • Accession Number:
      39409051